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在埃及对抗原特征循环牛性疹病毒亚型1.1的DIVA试验进行初步分子研究

Basel A Abukhadra¹, Sahar Abd El Rahman¹, Mohamed A Soltan²

¹Department of Virology, Faculty of Veterinary Medicine, Mansoura University, 35516 El Gomhoria Street, Mansoura, Egypt.

|February 17, 2024

概括

针对牛类alphaherpesvirus-1 (BoHV-1) 的标记疫苗旨在区分感染者和接种疫苗的动物 (DIVA). 这项研究发现,在患病的牛群中流传的疫苗病毒,可能会危及根除工作.

科学领域:

兽医病毒学兽医病毒学
分子诊断学分子诊断学
控制和控制疾病的疾病.

背景情况:

标志性疫苗对于在牛类αherpesvirus-1 (BoHV-1) 控制计划中区分感染者和接种疫苗的动物 (DIVA) 是至关重要的.
删除US2标记疫苗旨在区分接种疫苗的动物和感染野生型BoHV-1的动物.

研究的目的:

从接种疫苗的牛中选临床样本,以检测BoHV-1的呼吸系统症状.
为了分子表征和识别在该领域流通的潜在的US2-删除疫苗菌株.
评估基于US2基因的传统PCR DIVA试验的疗效.

主要方法:

多重实时PCR针对gD和gE基因进行BoHV-1查.
传统的PCR针对CG和US2侧边区域进行分子特征.
用于样本分类的遗传学分析.

主要成果:

在60个临床样本中,有6个对BoHV-1呈阳性.
传统的PCR DIVA试验显示,大多数BohV-1阳性样本缺乏US2基因,表明它们是疫苗菌株.
遗传学分析确定了循环的菌株为BoHV-1.1.1.

结论:

该研究确定了BoHV-1疫苗菌株在患病牛中的循环.
这一发现对正在进行的BoHV-1根除计划构成重大威胁.
根据流通的疫苗病毒,目前DIVA策略的有效性需要重新评估.

关键词:

这种病毒是BoHV-1病毒.传统的PCR是传统的PCR.迪瓦迪瓦迪瓦迪瓦多重复合RT-PCR是一种多重复合RT-PCR.独特的短2 (US2基因)

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相关概念视频

Herpes

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...