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对科丁D肠道微生物群代谢物的分析和活性验证

Weichen Xu¹, Shasha Han¹, Wenying Wang¹

¹Institute of Pediatrics, Jiangsu Key Laboratory of Pediatric Respiratory Disease, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China; Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.

Journal of pharmaceutical and biomedical analysis

|February 17, 2024

概括

肠道微生物群将Platycodin D,一种来自Platycodon grandiflorum的素,代谢成生物活性化合物. 这些代谢物,以及Platycodin D,表现出抗炎性质,尽管只有Platycodin D可以减少脂质积累.

科学领域:

药理学药理学是指药理学的学科.
微生物学微生物学
代谢学代谢学代谢学

背景情况:

普拉蒂科丁D是一种来自Platycodon grandiflorum的沙波宁,显示出抗肥胖的潜力.
口腔吸收不良需要了解其由肠道微生物群转化.
肠道微生物群将沙素转化为潜在的生物活性代谢产物.

研究的目的:

通过肠道微生物群对Platycodin D的代谢变化进行分析.
为了阐明Platycodin D的生物转化途径.
调查Platycodin D及其代谢产物的生物活性.

主要方法:

用小鼠肠道菌群化Platycodin D. 在小鼠肠道菌群中.
使用超高性能液体染色学-线性陷四极-轨道陷-质谱学 (UPLC-LTQ-轨道陷-MS) 的分析.
对抗炎症和降脂效应的评估.

主要成果:

确定了10种化合物,其中9种是Platycodin D的微生物代谢产物.
脱氧化和脱糖化是主要的代谢过程.
普拉提科丁D及其代谢物在LPS刺激的RAW 264.7细胞中显示出抗炎作用.
普拉提科丁D,但不是它的代谢物,减少了FFA治疗的HepG2细胞中的脂质积累.

结论:

通过肠道菌群生物转化Platycodin D的代谢概况得到了阐明.
普拉提科丁D及其代谢物具有抗炎活性.
普拉蒂科丁D的抗肥胖作用可能部分是由其对脂质积累的直接作用介导的.

关键词:

肠道的微生物群.代谢概况代谢概况普拉提科丁 D D UPLC-LTQ-轨道飞行器-MS 在

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