基于注射的毛发根诱导和植物再生技术在Brassicaceae中
Veronika Jedličková1, Marie Štefková1, Terezie Mandáková1,2
1Mendel Center for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Plant methods
|February 17, 2024
概括
这项研究为罕见的十字植物 (如Asperuginoides axillaris) 建立了高效的毛发根转化和再生协议. 这些毛的根为植物遗传和细胞遗传研究提供了稳定,持续的来源.
科学领域:
- 植物生物技术 植物生物技术
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
背景情况:
- 毛根是一种有价值的组织培养系统,用于繁殖难以生长的植物物种.
- 含有激素的介质可以促进转基因植物从毛的根源生成.
研究的目的:
- 为十字植物物种开发高效的毛发根转化和再生协议.
- 建立一个永久的植物材料来源,用于细胞遗传学研究.
主要方法:
- 使用Ri等离子体的Agrobacterium介导的毛发根诱导.
- 植物从已建立的毛的根系再生.
- 开发一个胚胎救援协议,以克服种子休眠状态.
主要成果:
- 在Asperuginoides axillaris,Arabidopsis thaliana和Cardamine hirsuta中成功形成毛的根和植物再生.
- 确认毛发根及其再生剂的遗传稳定性.
- 毛发根作为线粒染色体的来源的实用性.
结论:
- 对于A. axillaris,A. thaliana和C. hirsuta.的毛发根转化和再生,建立了有效的协议.
- 毛发根及其再生剂为分子和细胞遗传学分析提供了持续的植物材料供应.
关键词:
阿拉比多普西斯塔利亚纳.亚斯普鲁金诺伊德斯 (Aspuruginoides axillaris) 是一个状的动物.这种植物是Brassicaceae.卡尔达明 hirsuta 的使用情况克鲁西弗斯 (Crucifers) 是一个巨大的公司.细胞遗传学 细胞遗传学有毛的根,毛的根.植物再生 植物再生变化 转化 转化 转化更多相关视频
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