不同提取方法对Hericium coralloides多糖的结构和生物特性的影响
Zuo-Fa Zhang1, Guo-Ying Lv1, Ting-Ting Song1
1Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
Food chemistry
|February 18, 2024
概括
这项研究比较了Hericium coralloides多糖的九种提取方法,发现其特性和抗氧化活性存在显著差异. 单糖化合物组成和多醇含量影响了生物活性.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生化学
- 食品科学 食品科学 食品科学
背景情况:
- 来自Hericium coralloides (一种药用) 的多糖因其潜在的健康益处而闻名.
- 优化提取方法对于最大限度地提高这些有价值化合物的产量和生物活性至关重要.
研究的目的:
- 用九种不同的方法从Hericium coralloides中提取多糖.
- 为了比较提取的多糖的物理化学性质,结构特征和抗氧化活性.
- 确定影响Hericium coralloides多糖的生物活性的关键因素.
主要方法:
- 使用热反流,酸辅助,辅助,酶辅助,超声波辅助,冷水,加压热水,过氧化/酸和酸脱等方法提取多糖.
- 物理化学性质,化学成分,单糖成分和分子重量的表征.
- 使用ABTS,DPPH,OH和超氧化物激素清除试验对抗氧化剂活性的评估.
- 对L929细胞的细胞保护作用的评估.
主要成果:
- 在九种多糖提取物中,在提取产量,化学成分,单糖体配置和分子量方面观察到显著的差异.
- 通过热反流 (HRE-P) 提取的海里珊瑚化物多糖体显示出对ABTS和OH基的最高活性.
- 通过冷水提取的多糖 (CWE-P) 对ABTS,DPPH和超氧化基具有强烈的活性.
- 超声波辅助提取的多糖体 (UAE-P) 显示出显著的DPPH激素清除活性.
- 阿联-P,CWE-P和过氧化/酸提取的多糖化物 (HAE-P) 对L929细胞表现出显著的保护作用.
结论:
- 不同的提取方法产生了具有不同性质和生物活性的Hericium coralloides多糖.
- 单糖化合物的组成和总聚醇含量是这些多糖的抗氧化和保护作用的关键决定因素.
- 这项研究为选择最佳的提取策略提供了宝贵的见解,以获得具有所需功能的Hericium coralloides多糖.
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