在Pseudomonas putida中可持续的航空燃料前体异醇生产的基因组规模和途径工程
Deepanwita Banerjee1, Ian S Yunus1, Xi Wang1
1Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA, 94608, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Metabolic engineering
|February 18, 2024
概括
可持续航空燃料 (SAF) 的生产是使用工程 *Pseudomonas putida* *进行的. 这项研究优化了ISOPRENOL生物生产,这是一个关键的SAF前体,达到3.5g/L.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 可持续的航空燃料生产
背景情况:
- 可持续航空燃料 (SAF) 对于减少航空业对全球变暖的影响至关重要.
- 异二醇是SAF化合物1,4-二甲基环氧 octane (DMCO) 的重要前体.
- Pseudomonas putida 是因其利用植物生物质的能力而成为异二醇生物生产的有希望的宿主.
研究的目的:
- 为了改造Pseudomonas putida以提高异二醇的生产.
- 为了确定最佳的基因淘汰目标和代谢途径修改,以最大限度地提高异二醇产量.
- 为了证明P. putida在从可再生资源生产先进生物燃料方面的潜力.
主要方法:
- 伪虫的新陈代谢工程.
- 计算建模的应用,包括双层优化和受约束的最小切割集.
- 优化异烯酸铁酸盐 (IPP) - 绕道通路.
- 用于生产异二醇的料批发发酵.
主要成果:
- 成功地进行了P. putida的代谢工程,用于异二醇生物合成.
- 通过计算建模识别关键基因标.
- 获得了最高的异二醇生产标位3.5g/L.
- 通过使用可再生碳流来证明有效的生物生产.
结论:
- 工程P. putida是异二醇生物生产的可行的宿主.
- 计算机建模有效指导了应变工程的努力.
- 这种方法显著提高了生物质可持续航空燃料生产的潜力.
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