相关实验视频

Updated: Jul 2, 2025

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

增长分化因子-11通过诱导牛在人体外性热囊细胞中的表达来提高矩阵金属蛋白酶2的表达

Ze Wu¹, Qian Zhang¹, Hailong Wang¹

¹Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Molecular and cellular endocrinology

|February 18, 2024

概括

增长因子GDF-11通过上调调节人体 trofhoblast 细胞入侵增强. 牛通过GDF-11进行介绍.

科学领域:

生殖生物学生殖生物学
细胞和分子生物学是细胞和分子生物学.
发育生物学是发展生物学.

背景情况:

人类外性热囊细胞 (EVT) 细胞入侵对于正常的胎盘形成至关重要.
EVT细胞与癌细胞有着共同的迁移性和侵入性特征.
牛和牛的转录因子驱动上皮质-介质酶转换 (EMT),对入侵至关重要.

研究的目的:

调查GDF-11是否调节人类EVT细胞中的牛和牛表达.
确定牛和牛在GDF-11诱导的MMP2表达和EVT细胞入侵中的作用.

主要方法:

使用了人类EVT细胞系 (HTR-8/SVneo) 和初级人类EVT细胞培养.
研究了GDF-11对牛和牛表达的作用.
研究了ALK4/5和SMAD2/3信号通路的参与.
评估了GDF-11诱导的MMP2表达和细胞入侵.

主要成果:

在人类EVT细胞中,GDF-11显著提高了牛和牛表达的调节.
ALK4和ALK5调解GDF-11对牛和牛的刺激作用.
对于GDF-11诱导的牛表达,需要SMAD2和SMAD3;只有SMAD3可以调解slug表达.
牛,但不是 Slug,介导 GDF-11 诱导的 MMP2 表达和 EVT 细胞入侵.

结论:

GDF-11在调节人类EVT细胞入侵方面发挥着重要作用.
牛在MMP2表达和入侵的GDF-11信号通路中起着关键的调解作用.
这项研究阐明了一种通过GDF-11和Snail控制EVT细胞入侵的新机制.

关键词:

一切都是一样的 EVTT EVT 在GDF-11中使用.这是入侵的入侵.这就是MMP2的MMP2.一个牛牛牛.

更多相关视频

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...