植物王国的有机纳米纤维与人类细胞生理学之间的联系:细胞内信号传递
Martina Trentini1, Ilaria Zanolla2, Elena Tiengo1
1Department Translational Medicine, University of Ferrara, 44121, Ferrara, Italy.
Journal of nanobiotechnology
|February 18, 2024
概括
果衍生的纳米微粒 (ADNV) 通过信号传递在人体细胞中表现出抗氧化作用. 这些植物性纳米纤维含有生物活性蛋白,小RNA和脂质,有机来源产生更多的DNAV.
科学领域:
- 植物生物学和纳米医学
- 生物化学和细胞信号传递
背景情况:
- 植物衍生纳米纤维 (PDNVs) 正在医学和农业食品领域出现.
- 果 (Malus domestica) 是PDNV (ADNV) 的一个有希望的来源,由于消费和浪费.
- DNAV与人体细胞相互作用,诱导抗氧化和抗炎反应.
研究的目的:
- 通过蛋白学,脂学和小RNA测序来生物化学地表征DNAVs.
- 通过信号传导阐明DNAVs在人体细胞中的作用机制 (MOA).
- 为了比较综合和有机果生产中的DNAV含量.
主要方法:
- 对DNAVs的蛋白质组和脂质组分析.
- 在DNAVs的小RNA测序.
- 在人体纤维细胞中进行细胞内 (Ca2+) 信号测试.
主要成果:
- DNAVs由外囊阳性有机体 (EXPOs) 和外体样囊泡组成.
- DNAV含有参与植物保护的蛋白质和miRNAs (ABA信号传递,ROS代谢).
- DNAVs诱导细胞内Ca2+峰值,并在人体细胞中表现出抗氧化作用;有机DNAVs富含溶解脂.
结论:
- 通过Ca2+信号通路,ADNV对人类细胞产生抗氧化作用.
- ADNV含有各种生物活性分子,在植物生物学中具有已知的功能.
- 在DNAV中含有的植物性蛋白质可能具有对人体细胞有益的抗氧化特性.
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