相关实验视频

Updated: Jul 2, 2025

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Published on: May 15, 2019

在穿孔吸食者和食草食者之间对比植物转录组反应,超出了感染地点

Álvaro Montesinos^1,2, Soledad Sacristán^1,3, Palmira Del Prado-Polonio¹

¹Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC) Campus de Montegancedo, Pozuelo de Alarcón, 28223, Madrid, Spain.

BMC plant biology

|February 18, 2024

概括

草食动物的养策略会影响植物的防御反应,影响受感染和未受感染的组织. 了解这些独特的植物反应对于管理生物压力至关重要.

科学领域:

植物科学植物科学
生态生态学生态生态学
分子生物学分子生物学

背景情况:

植物拥有针对草食动物的多种防御机制,这些机制是由斯酸 (JA) 和酸 (SA) 等激素调节的.
植物对草食的反应可以扩展到非感染的组织,受草食者的食策略的影响.

研究的目的:

为了研究不同的食草动物食策略 (与刺口吸) 如何不同地触发Arabidopsis thaliana的植物防御反应.
分析感染和远端组织中的转录和激素变化.

主要方法:

阿拉比多普西斯·塔利亚纳 (Arabidopsis thaliana) 的花束被皮埃里斯·布拉西卡 (Pieris brassicae) () 或特拉尼克斯·乌尔蒂卡 (Tetranychus urticae) (穿孔吸) 感染.
进行了转录组分析,以评估基因表达变化对感染的反应.
在受感染和根组织中检查了荷尔蒙调节,黄路径和细胞壁重组.

主要成果:

皮埃里斯棕虫感染诱导了涉及压力基因和JA/酸 (ABA) 调节的短期反应.
刺虫 (Tetranychus urticae) 感染引发了长期的,较低幅度的转录组反应,特别影响了SA调节.
在根部观察到明显的防御反应,包括差异激素调节和细胞壁修饰.

结论:

草食动物的食策略显著塑造了植物的转录和荷尔蒙防御反应.
这些防御反应是系统性的,影响非感染的组织,甚至根.
考虑整个植物的反应对于理解植物-食草动物相互作用至关重要.

关键词:

阿拉比多普西斯塔利亚纳.叶子叶子叶子植物虫的感染.巴西的皮埃里斯 (Pieris brassicae) 是一个根系根系根系根系一个叫做Tetranychus urticae的小虫.

更多相关视频

Herbivore-induced Blueberry Volatiles and Intra-plant Signaling

Herbivore-induced Blueberry Volatiles and Intra-plant Signaling

Published on: December 18, 2011

Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example

Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example

Published on: June 19, 2011

相关概念视频

Defenses Against Pathogens and Herbivores

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.

Epiphytes, Parasites, and Carnivores

Epiphytes, Parasites, and Carnivores

Plants often form mutualistic relationships with soil-dwelling fungi or bacteria to enhance their roots’ nutrient uptake ability. Root-colonizing fungi (e.g., mycorrhizae) increase a plant’s root surface area, which promotes nutrient absorption. While root-colonizing, nitrogen-fixing bacteria (e.g., rhizobia) convert atmospheric nitrogen (N2) into ammonia (NH3), making nitrogen available to plants for various biological functions. For example, nitrogen is essential for the...

Responses to Gravity and Touch

Responses to Gravity and Touch

Gravitropism: Plant Responses to Gravity

Pollination and Flower Structure

Pollination and Flower Structure

Flowers are the reproductive, seed-producing structures of angiosperms. Typically, flowers consist of sepals, petals, stamens, and carpels. Sepals and petals are the vegetative flower organs. Stamens and carpels are the reproductive organs.