相关实验视频

Updated: Jul 2, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Compositae-ParaLoss-1272:一个补充的日特异性探针集减少了复杂系统的基因组学分析中的对比

Erika R Moore-Pollard¹, Daniel S Jones², Jennifer R Mandel¹

¹Department of Biological Sciences University of Memphis 3700 Walker Ave. Memphis Tennessee 38152 USA.

Applications in plant sciences

|February 19, 2024

概括

一个新的探针组,Compositae-ParaLoss-1272,通过减少类似序列,显著提高了向日家族的基因组分辨率. 这一进步有助于在较低的分类层研究复杂的植物群.

科学领域:

植物学植物学
基因组学就是基因组学.
进化生物学进化生物学

背景情况:

太阳花家族 (Asteraceae) 的基因组学研究受到多化和杂交的挑战.
现有的探针集,如Compositae-1061,在属和物种层面上可能缺乏分辨率.

研究的目的:

开发和评估一个新的Hyb-Seq探针集 (Compositae-ParaLoss-1272),以改善Asteraceae的基因组学分析.
评估探头组的效率和有效性,特别是在像Packera这样的复杂品种中.

主要方法:

在基模拟的目标丰富序列.
包括针对Asteraceae的正统位置.
在19个 *Packera* 种群上使用 Compositae-ParaLoss-1272 的 Hyb-Seq 实验.
遗传学分析比较探头组及其组合.

主要成果:

Compositae-ParaLoss-1272在测试的Asteraceae成员中成功捕获了位点.
与Compositae-1061.1相比,新的探针组显示出较少的基因树异调,并保留了较长的基因位点.
复合物-ParaLoss-1272恢复的相似序列明显少 (~5%) 与复合物-1061 (~59%) 相比.

结论:

Compositae-ParaLoss-1272为日家族的基因组学研究提供了增强的分辨率和实用性.
探针组对于研究复杂的群体和较低的分类层面特别有益.
它解决了在植物的植物基因组分析中 Orthology 赋值的挑战.

关键词:

阿斯特拉属植物 (Asteraceae) 是一个植物.在 Hyb-Seq 里.标记器Miner的标记器这是一个包装.通过双重捕获的方式捕获数据.多重积分多样性多重积分多样性目标是丰富的目标.

更多相关视频

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

相关概念视频

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...