相关实验视频
Updated: Jul 2, 2025

10:53
Microgavage of Zebrafish Larvae
Published on: February 20, 2013
17.0K
对于ZEB1在调节antrochoanal多体紧接处中的作用
Yisha Wu1,2, Dingqian Hao1, Yanyi Tu1
1Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Jinan, Shandong, China.
Heliyon
|February 19, 2024
概括
安特罗阿纳尔多体显示严重损坏的紧密结节,与疾病严重程度有关. 受IL-17A影响的转录因子ZEB1在这种损伤中起着关键作用,影响鼻上皮质屏障.
科学领域:
- 鼻腔病理学 鼻腔病理学
- 皮质生物学 皮质生物学
- 疾病的分子机制.
背景情况:
- 安特罗阿纳尔多 (ACP) 是一种良性鼻腔质,病因不明.
- 紧结 (TJs) 构成一个重要的上皮屏障,但它们在ACP中的作用尚不清楚.
研究的目的:
- 为了研究在ACP的紧密结合的表型.
- 探索ZEB1和IL-17A在非洲和非洲的病原性中的作用.
主要方法:
- 在ACP中分析TJ标记物 (ZO-1,Claudin-3,Occludin) 和ZEB1,慢性鼻炎与鼻 (CRSwNP),以及使用qRT-PCR和免疫光检测的对照组织.
- 在人鼻上皮细胞 (hNEpCs) 中涉及ZEB1操纵 (过度表达和沉默) 和IL-17A刺激的体外实验.
主要成果:
- 与对照组相比,ACP组织表现出ZO-1,奥克卢丁和克劳丁-3的显著下调,严重程度与TJ中断相关.
- 在ACP中,ZEB1,IL-17A和IFN-γ的调控得到了提高.
- 过度表达ZEB1会损害TJ标记物,而ZEB1沉默会增强屏障功能.
- IL-17A刺激破坏了TJs,这种效应通过与si-ZEB1.1的同时治疗而逆转.
结论:
- 紧张的结点在ACP严重受损,与疾病严重程度相关.
- ZEB1参与了ACP的发病过程,它调解了IL-17A对紧结的作用.
更多相关视频
相关概念视频
Tight Junctions
5.3K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.3K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Adherens Junctions
4.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.8K
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K

