在发酵的Benincasa hispida中识别标记化合物并验证其分析方法
Sun-Il Choi1, Xiao Men2, Geon Oh2
1Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Republic of Korea.
Food chemistry: X
|February 19, 2024
概括
贝尼卡萨希斯皮达的发酵通过识别关键的生物活性化合物,如2 - 酸,来标准化. 这一过程提高了其作为功能性食品成分的潜力.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 发酵通过增加生物活性化合物来增强健康功能.
- 为了获得一致的功能益处,对Benincasa hispida的发酵标准化至关重要.
研究的目的:
- 为了识别和量化本尼卡萨西皮达发酵过程中产生的标记化合物.
- 为了验证一种分析方法来评估这些标记物化合物.
主要方法:
- 超高性能液体染色学-四极飞行时间质谱学 (UPLC-QTOF-MS/MS) 用于化合物识别.
- 使用光二极管阵列 (HPLC-PDA) 的高性能液态染色学用于内容分析和方法验证.
主要成果:
- 确定了2-furoic酸,2,3-dihydroxybenzoic酸和rubinaphthin A作为标记化合物.
- 在发酵后证实了这些化合物的增加水平.
- 验证了分析方法的特异性,线性,LOD/LOQ,精度和准确性.
结论:
- 这项研究确定了Benincasa hispida.的标准化发酵过程.
- 确定了关键标志物化合物和验证的质量控制分析方法.
- 这些发现支持发酵Benincasa hispida作为功能性食品成分的发展.
更多相关视频
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
3.9K
07:29HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
2.0K
相关概念视频
Differential Staining Technique
2.7K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.7K
Methods of Classification and Identification
2.3K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
2.3K
