LncRNA PRKCA-AS1促进LUAD进展,并通过海绵miR-508-5p调节S100A16作为ceRNA的功能
Chaohui Wu1, Jiansheng Yang1, Xianbin Lin1
1Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, 362000, China.
Journal of Cancer
|February 19, 2024
概括
长非编码RNAPRKCA-AS1通过海绵化miR-508-5p和上调S100A16.1促进肺腺癌 (LUAD) 的进展. 向PRKCA-AS1为LUAD提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 肺腺癌 (LUAD) 是癌症相关死亡的主要原因.
- 了解推动LUAD进展的分子机制对于开发有效疗法至关重要.
- 长非编码RNAs (lncRNAs) 已经成为各种癌症的关键调节者,包括LUAD.
研究的目的:
- 研究lncRNA PRKCA-AS1在LUAD进展中的作用和机制.
- 阐明在LUAD中涉及PRKCA-AS1,miR-508-5p和S100A16的监管网络.
- 评估PRKCA-AS1作为LUAD治疗点的潜力.
主要方法:
- 定量实时PCR (qRT-PCR) 用于分析PRKCA-AS1,miR-508-5p和S100A16.1的表达水平.
- 在PRKCA-AS1倒退或过度表达后进行细胞增殖和转移测定.
- 双露西法酶记者测定和RNA免疫沉 (RIP) 来确认分子相互作用.
- 在裸体小鼠的体内瘤生成实验.
主要成果:
- 在LUAD组织中,PRKCA-AS1和S100A16显著上调,而miR-508-5p则下调.
- 抑制PRKCA-AS1抑制了LUAD细胞的增殖和转移;相反,miR-508-5p抑制或S100A16过度表达促进了这些过程.
- PRKCA-AS1通过菌miR-508-5p作为竞争的内源RNA (ceRNA) 起作用,从而增加S100A16表达并促进LUAD进展.
结论:
- 通过调节miR-508-5p/S100A16轴,LncRNA PRKCA-AS1在LUAD中起着致癌作用.
- PRKCA-AS1作为一个ceRNA,在体外和体内都能调节LUAD进展.
- PRKCA-AS1代表了LUAD治疗的一个有前途的治疗标.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:07Tumor Progression
6.3K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.3K
01:22PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K