等离子体调节电路的进化 改善等离子体适应性 成本
Clinton A Elg1,2,3, Erin Mack3, Michael Rolfsmeier3
1Bioinformatics and Computational Biology Program, University of Idaho, Moscow, Idaho, USA.
bioRxiv : the preprint server for biology
|February 19, 2024
概括
一个等离子体基因,upf31,阻碍了它在大肠杆菌中的维护. 通过改变基因调节,禁用该基因可以改善等离子体的稳定性,这表明单基因进化如何影响细菌等离子体宿主范围.
科学领域:
- 微生物学 微生物学
- 细菌遗传学 细菌遗传学
- 分子生物学分子生物学
背景情况:
- 等离子体是通过基因转移促进细菌适应和抗生素耐药性的关键驱动因素.
- 广域宿主等离子体通常在各种细菌物种中存在,但也有例外.
- 一些等离子体在新宿主中的不良持久性仍然不太了解.
研究的目的:
- 研究实验室大肠杆菌中广域宿主等离子体pBP136的灭绝情况.
- 确定导致特定细菌宿主体等离子体不稳定的遗传因素.
- 阐明塑体宿主范围限制背后的分子机制.
主要方法:
- 含有等离子体的大肠杆菌种群的实验进化.
- 基因操纵,包括基因失活 (upf31) 和基因表达 (转换).
- 质粒和染色体基因的转录分析 (RNA测序).
- 蛋白质与蛋白质相互作用的无分析 (Upf31和KorB).
主要成果:
- 塑基因upf31的非激活显著增强了E. coli中的pBP136维护.
- upf31无活化减少了等离子体调节剂 (korA,korB,korC) 和它们的标的转录.
- upf31表达对大肠杆菌中pBP136和相关等离子体R751的稳定性产生了负面影响.
结论:
- 质粒基因upf31在pBP136在大肠杆菌中的持久性中起着不利的作用.
- Upf31与等离子体调节者的相互作用可能会对其对等离子体维护的负面影响产生影响.
- 单个等离子体基因的进化变化,如upf31,通过调节基因表达和调节,可以显著改变等离子体的宿主范围.
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