相关实验视频

Updated: Jul 2, 2025

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

删除omeTools:使用酵母删除菌株转录组汇编来识别协同功能基因

Maxwell L Neal¹, Sanjeev K Choudhry¹, John D Aitchison^1,2

¹Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States.

bioRxiv : the preprint server for biology

|February 19, 2024

概括

DeleteomeTools是一个R包,通过分析酵母基因删除转录组来预测基因功能. 它确定了核孔复合体和DNA复制机械之间的新联系,经过实验验证.

科学领域:

酵母遗传学酵母遗传学
系统生物学系统生物学
计算生物学是一种计算生物学.

背景情况:

了解基因功能对于生物研究至关重要.
删除组汇编提供全基因组的酵母单基因删除转录组.
需要预测工具来利用这些数据用于功能基因组学.

研究的目的:

介绍DeleteomeTools,一个R包,用于使用Deleteome汇编预测基因功能.
为了证明包装能够根据转录组签名识别基因之间的功能关系的能力.
通过实验验证软件所做的新型基因功能预测.

主要方法:

使用酵母单基因删除转录组的Deleteome汇编.
在R包中开发功能,以识别删除菌株的转录基因特征的相似性.
使用差异表达特征来量化菌株相似性.
预测基因功能关系的实验验证.

主要成果:

DeleteomeTools成功地预测了酵母核素Nup170和Ctf18-RFC复合体之间的新功能关系.
这一预测得到了实验证实,揭示了核孔综合体与DNA复制之间的新联系.
该包准确预测了大多数现有的基因本体学 (GO) 生物过程注释.

结论:

删除omeTools是一个有效的R包,用于预测酵母中的基因功能.
使用微分表达签名的方法为发现功能关系提供了一种独特的方法.
该软件有可能在生物系统中发现以前未被检测到的功能链接.

更多相关视频

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

相关概念视频

Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...