标准的动物饮食对酒精消费和偏好以及肠道微生物群多样性有不同的影响
Aline Zaparte1, Evan Dore2, Selby White2
1Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans.
bioRxiv : the preprint server for biology
|February 19, 2024
概括
动物的饮食显著影响小鼠的酒精消费和偏好,影响AUD模型. 改变饮食迅速改变摄入量,这表明与肠道微生物组变化和强迫性饮酒行为有关.
科学领域:
- 神经科学是一个神经科学.
- 动物模型 动物模型
- 微生物组研究 微生物组研究
背景情况:
- 酒精使用障碍 (AUD) 是一个重要的健康问题,几乎没有有效的治疗方法.
- 临床前模型对于理解AUD至关重要,但酒精摄入量的变化带来了可重现性挑战.
- 动物的饮食成分越来越被认为是影响研究环境中酒精消费的因素.
研究的目的:
- 为了研究不同的商用动物饮食如何影响C57BL/6J小鼠的酒精消费和偏好.
- 为了确定饮食变化是否能快速改变酒精摄入量.
- 探索饮食对强迫性饮酒和肠道微生物组的潜在影响.
主要方法:
- 在C57BL/6J小鼠中使用了24小时间歇性访问程序.
- 在三种不同的动物饮食配方 (LabDiet 5001,LabDiet 5053,Teklad 2019S) 中比较了酒精消耗和偏好.
- 使用糖,糖和来评估味觉感知;分析肠道微生物组的组成.
主要成果:
- 实验室饮食5001导致了最高的酒精消费和偏好,其次是实验室饮食5053和Teklad 2019S.
- 在服用酒精前48小时的饮食改变迅速改变了饮酒消费和偏好.
- 在被食LabDiet 5001的小鼠中观察到氨酸耐性酒精摄入量的增加,这表明对强迫性饮酒的潜在影响.
- 在饮食组之间发现了肠道微生物群多样性 (α和β多样性) 的显著差异.
结论:
- 动物饮食配方是影响AUD临床前模型中酒精消费和偏好的关键变量.
- 饮食干预可以快速调节酒精摄入量,提供一种标准化动物模型的潜在策略.
- 饮食引起的肠道微生物组变化可能在通过肠-大脑轴调节酒精消费和强迫性行为方面发挥作用.
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