Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Regulated mRNA Transport02:22

Regulated mRNA Transport

6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Reporter Genes02:11

Reporter Genes

11.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K
DNA Microarrays02:34

DNA Microarrays

17.4K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.4K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

[The role of mitochondrial gluconeogenesis in de novo serine synthesis in mouse hepatocytes under high-selenium conditions].

Wei sheng yan jiu = Journal of hygiene research·2026
Same author

Context-dependent regulation of IgH V(D)J recombination by cohesin-STAG1 and cohesin-STAG2.

Nature communications·2026
Same author

Chemoimmunotherapeutic sealants for postsurgical adjuvant therapy of malignant tumors.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Genome evolution through polyploidy: Enhancing plant stress resilience in agriculture.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Biogas production and microbial profile estimation in bioreactor landfills.

Frontiers in chemistry·2026
Same author

FTMT-mediated suppression of mitophagy links iron accumulation to osteoporosis.

Redox biology·2026

相关实验视频

Updated: Jul 2, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K

空间变量基因检测在空间转录组学中的最新进展

Sikta Das Adhikari1,2, Jiaxin Yang1, Jianrong Wang1

  • 1Department of Computational Mathematics, Science and Engineering, Michigan State University, East Lansing, MI 48824, USA.

Computational and structural biotechnology journal
|February 19, 2024
PubMed
概括

本综述涵盖了在空间转录组学数据中识别空间变量基因 (SVG) 的方法. 它强调了SVG对生物学理解和下游分析的重要性.

关键词:
一个单细胞RNA测序.空间转录组学 空间转录组学空间分辨的转录学.空间变量的基因

更多相关视频

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

2.7K
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

7.2K

相关实验视频

Last Updated: Jul 2, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

2.7K
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

7.2K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 空间转录学技术使得在组织背景下进行基因表达分析成为可能.
  • 识别空间变量基因 (SVGs) 是空间转录组学数据分析的关键第一步.
  • SVG揭示了组织架构和细胞功能.

研究的目的:

  • 为检测空间变量基因 (SVGs) 提供当前方法的选择性审查.
  • 讨论对SVG检测文献的实际实施和见解.
  • 帮助研究人员理解和应用SVG检测技术.

主要方法:

  • 空间转录学分析方法的文献综述.
  • 专注于识别具有特定空间表达模式的基因的技术.
  • 主要SVG检测方法的选择性策划.

主要成果:

  • 越来越多的SVG检测方法已经出现.
  • 围绕SVG识别的各种创新概念和讨论.
  • 审查提供了关于这些方法的实际实施的见解.

结论:

  • SVG检测对于从空间转录组学推进生物见解至关重要.
  • 该领域正在迅速发展,有新的方法和概念.
  • 本综述是对SVG检测技术当前环境的指南.