从使用玉米作为诱导基质 CM1 的印尼本地转基因 Bacillus halodurans CM1 来共同生产性蛋白酶和西兰酶
I Gede Eka Perdana Putra1, Maria Ulfah2, Niknik Nurhayati2
1Research Center for Applied Microbiology, National Research and Innovation Agency, Republic of Indonesia (BRIN). Jalan Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, Jawa Barat 16911, Indonesia.
Saudi journal of biological sciences
|February 19, 2024
概括
玉米废料可以用于同时生产有价值的工业酶,蛋白酶和西兰酶. 这项研究优化了酶共产的条件,显示了洗剂添加剂的潜力.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 工业微生物学 工业微生物学
背景情况:
- 农业工业废物,特别是玉米,面临着处置方面的挑战.
- 将废物流转化为有价值的产品是可持续发展的一个关键目标.
- 酶生产通常需要特定的基质和优化的条件.
研究的目的:
- 为了研究从玉米废物中蛋白酶和西兰酶的共产.
- 为了优化玉米的度,同时进行酶合成.
- 为了描述产生的热友蛋白酶的特性.
主要方法:
- 使用了B.的重组菌株. 杜兰CM1用于酶生产.
- 研究了玉米集中在生物反应器中的影响.
- 在各种条件下评估蛋白酶和西兰酶活动.
- 在不同的pH值,温度和化学环境下特征蛋白酶稳定性和活性.
主要成果:
- 玉米有效地作为碳源和诱导蛋白酶和西兰酶生产.
- 玉米的最佳度 (6%) 产生了高的酶活性 (蛋白酶:1020.7 U/mL,西兰酶:502.8 U/mL).
- 产生的蛋白酶是一种热性血清蛋白酶,在pH12和50°C活跃,在有机溶剂和洗剂中稳定.
结论:
- 玉米是一种可行的基质,可用于节省成本的蛋白质酶和西兰酶的联合生产.
- 具有特征的热友蛋白酶显示出在洗剂和其他工业过程中的应用的前景.
- 这种方法为废物利用和酶制造提供了一种可持续的方法.
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