通过Bacillus methylotrophicus在固态发酵中共同生产脂酶和生物表面活性剂
Naiara Elisa Kreling1, Victória Dutra Fagundes1, Viviane Simon1
1Institute of Technology, Post-graduation Program in Civil and Environmental Engineering, Universidade de Passo Fundo, Campus I, L1 Building, BR 285, Bairro São José, 611, Passo Fundo, RS CEP: 99052-900 Brazil.
3 Biotech
|February 19, 2024
概括
这项研究优化了使用Bacillus methylotrophicus通过固态发酵 (SSF) 的生物表面活性剂和脂酶生产. 发酵基质有效地修复了生物柴油污染的土壤,显示出显著的生物降解和降低的表面张力.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 微生物学 微生物学
背景情况:
- 通过固态发酵 (SSF) 生产生物活性剂和脂酶在细菌应用中尚未得到充分研究.
- 通过简化生物复合物回收,SSF提供了生物修复的潜力.
- 甲基菌 (Bacillus methylotrophicus) 正在研究同时生产生物复合物.
研究的目的:
- 使用Bacillus methylotrophicus通过SSF.优化生物表面活性剂和脂酶同时生产的条件.
- 为了评估发酵基质在修复被生物柴油污染的土壤中的有效性.
- 评估生物化合物,生物刺激和生物增量在土壤生物修复中的作用.
主要方法:
- 固态发酵 (SSF) 使用Bacillus methylotrophicus与优化的基质混合物 (小麦/玉米),水分,甘油,和糖蜜.
- 确定8天内生物表面活性剂 (降低表面张力) 和脂酶活性的最佳发酵条件.
- 发酵基质的应用,用于90%生物柴油污染的粘土土的90天生物修复.
主要成果:
- 在最佳的SSF条件下,表面张力降低24.61%,脂酶活性降低3.54U/mL.
- 生物柴油污染土壤的生物修复在60天内实现了72.08%的生物降解.
- 发酵基质作为生物刺激剂/生物增量剂降低了23.97%的土壤表面张力,并在现场产生1.52 U/mL的脂酶.
结论:
- 固态发酵是Bacillus methylotrophicus同时生产生物表面活性剂和脂酶的有效方法.
- 发酵基质显示出生物柴油污染土壤生物修复的巨大潜力.
- 在土壤中的现场生物化合物生产通过生物刺激和生物增强提高了生物降解效率.
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