用白菜废料作为微生物多甲基酸盐生产的原料:优化水解条件和多甲基酸盐生产
Jung Eun Yang1, Hye Sung Jeon1, Seulbi Kim1,2
1Technology Innovation Research Division, World Institute of Kimchi, 86 Kimchi-ro, Nam-gu, Gwangju 61755, Republic of Korea.
Journal of agricultural and food chemistry
|February 19, 2024
概括
这项研究利用大肠杆菌 (Escherichia coli) 来从卷心菜废物中产生多基酸盐. 优化条件和酸补充剂显著提高了聚3-基酸) 的产量,显示出废物回收利用潜力.
科学领域:
- 生物技术是生物技术.
- 可持续化学 可持续化学
- 微生物发酵 微生物发酵
背景情况:
- 废物的生物转化为增值化合物对于可持续经济至关重要.
- 聚酸酸盐 (PHAs) 是一种可生物降解的聚合物,具有多种应用.
- 为PHA生产开发具有成本效益的原料是一个持续的挑战.
研究的目的:
- 建立一个平台,从卷心菜废物中生产聚酸酸盐 (PHA).
- 通过代谢工程设计大肠杆菌以增强PHA生物合成.
- 为了优化卷心菜废物水解和发酵条件.
主要方法:
- 通过调整基质和酶度,优化了白菜废物的水解.
- 在大肠杆菌中过度表达了来自Ralstonia eutropha H16的phaABC操作子.
- 使用的白菜水解剂作为微生物发酵的原料.
主要成果:
- 工程化大肠杆菌产生了聚3-基酸盐 (PHB),其聚合物含量为干细胞重量的26.0 wt %.
- 卷心菜水解剂中的酸显著增强了PHB的产生.
- 添加0.5克/升酸增加了PHB含量59.9%.
结论:
- 卷心菜废料是微生物PHA生产的可行和有前途的原料.
- 大肠杆菌的代谢工程和过程优化可以提高PHA产量.
- 这种方法有助于废物利用和循环经济的发展.
相关概念视频
Microbes in Food Production
308
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
308
Microbes in the Production of Fermented Foods
247
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
247
Production of Organic Acids
82
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
82
Bioplastics
50
Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
50
Biofuels
92
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
92
Microbial Bioremediation of Plastics
93
Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
93


