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相关概念视频

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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相关实验视频

Updated: Jul 2, 2025

Author Spotlight: Efficient Detection of Immune Cell-Infiltration in Cancer Tissues Using Fluorescent Immunohistochemistry
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多复合循环光免疫组织化学

Yun Chen1, Hui Zhang2, Yaodong Fan3

  • 1Department of Pathology, The Third Affiliated Hospital of Kunming Medical University & Yunnan Cancer Hospital.

Journal of visualized experiments : JoVE
|February 19, 2024
PubMed
概括

多重循环光免疫组织化学可以同时检测组织样本中的多个标记物. 这种可复制的技术有助于分析免疫细胞子集,用于免疫疗法研究和临床应用.

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科学领域:

  • 免疫学 免疫学 免疫学
  • 病理学 病理学 病理学
  • 生物技术是生物技术.

背景情况:

  • 瘤微环境 (TME) 包含了对疾病进展和治疗反应至关重要的复杂相互作用.
  • 在TME中表征免疫细胞子集和蛋白质表达对于预后和治疗策略至关重要.
  • 多重复合免疫组织化学技术已经出现,以解决同时检测标记物的需要.

研究的目的:

  • 描述多重循环光免疫组织化学 (mcf-IHC) 的工作流程.
  • 为了证明mcf-IHC在量化淋巴细胞子集中的应用.
  • 突出mcf-IHC在免疫疗法研究和临床应用中的价值.

主要方法:

  • 为mcf-IHC开发了一种工作流程,使用甲固化嵌入 (FFPE) 组织幻灯片.
  • 该协议涉及抗原检索,循环抗体化和染色,类似于标准免疫组织化学.
  • 针对抗原检索和抗体度进行了优化,以提高信号噪声比.

主要成果:

  • 该研究成功建立并验证了一个可重复的mcf-IHC工作流.
  • 该试验允许同时检测和量化多个标记物,包括淋巴细胞子集.
  • 优化条件改善了信号与噪声的比率,从而实现了准确的分析.

结论:

  • 多重循环光免疫组织化学是一种有价值和可重复的工具,用于详细的TME分析.
  • 这种技术有助于全面了解细胞功能和细胞间相互作用.
  • mcf-IHC具有促进免疫治疗研究和临床诊断的巨大潜力.