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Ресвератрол通过Nrf2/HO-1通路减轻肠上皮质屏障功能障碍,在硫酸诱导的Caco-2细胞中

Xinya Yu¹, Yazhi Wang¹, Yunchun Xu¹

¹Department of Medical Microbiology and Immunology, School of Basic Medical Sciences, Dali University, Dali, Yunnan, People's Republic of China.

Immunity, inflammation and disease

|February 19, 2024

概括

复星通过减少炎症和增强紧密结合蛋白来增强肠道屏障功能. 它激活Nrf2/HO-1通路,为早期结肠炎提供治疗潜力.

科学领域:

胃肠病学胃肠病学
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

由肠上皮细胞 (IEC) 和它们的紧密结节 (TJ) 维持的肠壁对于防止病原体进入至关重要.
肠道屏障功能障碍与各种炎症性疾病有关.
植物化合物白醇 (Res) 具有抗炎性质.

研究的目的:

调查resveratrol在增强肠上皮细胞 (IEC) 屏障功能的有效性.
阐明了背后的分子机制复星对屏障功能障碍的保护作用.

主要方法:

硫酸德克斯 (DSS) 用于诱导IECs的肠壁功能障碍.
与酶相关的免疫吸收试验 (ELISA) 量化了炎症性细胞因子.
经皮质电阻 (TEER) 和西部斑点分析评估了屏障完整性和紧结蛋白表达 (ZO-1,Occludin).
网络药理学确定了关键目标和途径,并通过Western blot进行了验证.

主要成果:

通过减少IL-6,IL-1β和TNF-α,Resveratrol减轻了炎症,同时增加了IL-10.
TEER值有所改善,ZO-1和奥克卢丁的表达被Resveratrol上调.
网络药理学将NFE2L2 (Nrf2) 确定为主要标;复星增加了Nrf2和血氧酶1 (HO-1) 蛋白质水平.

结论:

复星可以改善DSS引起的肠壁功能障碍.
保护作用是由Nrf2/HO-1通路的激活介导的.
Ресвератрол在早期结肠炎中显示出治疗潜力.

关键词:

Nrf2 / HO-1 的一个.肠道屏障的功能肠道屏障的功能肠道上皮细胞肠道上皮细胞复星醇是一种复星醇.紧密的交叉路口紧密的连接

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