通过工程改善微生物细胞工厂的性能SAM可用性SAM可用性
Yongkun Lv1, Jinmian Chang1, Weiping Zhang2
1School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou 450001, China.
Journal of agricultural and food chemistry
|February 19, 2024
概括
在微生物细胞工厂 (MCFs) 中可持续生产SAM依赖的自然产品 (SdNPs) 需要优化S-Adenosyl-l-methionine (SAM) 的可用性. 本综述探讨了SAM生物合成,调节和替代甲基来源,以提高MCF的性能.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 自然产品的合成自然产品的合成
背景情况:
- 甲基化天然产品是多样化和至关重要的,其中S-Adenosyl-l-methionine (SAM) 是主要的甲基供体.
- 微生物细胞工厂 (MCF) 越来越多地用于生产依赖SAM的天然产品 (SdNP).
- 有限的SAM可用性和低效的甲基供应阻碍了MCF中的SdNP生产.
研究的目的:
- 在MCF开发的背景下,审查有关SAM生物合成和调节的当前知识.
- 识别和评估可持续的甲基来源,以改善SAM的可用性.
- 讨论代谢工程策略和基因电路设计,以提高SdNP的生产.
主要方法:
- 关于SAM生物合成途径和调节机制的文献综述.
- 对不同的甲基组来源进行比较分析,包括一碳化合物.
- 检查SAM池的代谢工程方法和动态调节策略.
主要成果:
- SAM生物合成受到严格监管,并与细胞能量池 (ATP,NADPH) 联系在一起.
- 替代的甲基来源,特别是廉价的单碳化合物,显示出可持续生产的前景.
- 代谢工程和动态调节提供了可行的策略来提高细胞内SAM水平.
结论:
- 优化甲基源供应和SAM生物合成调节对于MCF中高效的SdNP生产至关重要.
- 工程SAM响应性遗传电路具有在MCF中进行先进控制的潜力.
- 通过综合代谢工程方法可以实现有价值的SdNP的可持续生产.
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