贝叶斯细胞疗法过程优化和优化
Evan Claes1,2, Tommy Heck1, Kathleen Coddens1
1Antleron, Leuven, Belgium.
Biotechnology and bioengineering
|February 19, 2024
概括
贝叶斯优化 (BO) 通过优化复杂的生物过程来增强细胞疗法制造. 这种方法显著减少了所需的实验,超过了当前技术,以实现更快,更高效的过程开发.
科学领域:
- 生物技术是生物技术.
- 工艺工程是过程工程.
- 细胞疗法制造业 细胞疗法制造业
背景情况:
- 优化复杂的生物过程,特别是在细胞疗法中,由于有限的生物学理解和资源密集型实验,具有挑战性.
- 开发定制,封闭和自动化流程对于工业翻译和成本有效的患者治疗至关重要.
- 贝叶斯优化 (BO) 是一种实验性设计方法,但其在杂,并行生物过程优化中的应用需要进一步调查.
研究的目的:
- 为了评估噪音,平行贝叶斯优化 (BO) 对生物过程优化的性能.
- 评估增加噪音水平和并行批量大小对BO有效性的影响.
- 将拟议的BO方法与当前行业的最新情况进行比较.
主要方法:
- 在两种生物工艺模型上对杂的平行BO进行in silico评估.
- 使用不同噪声水平和平行批量大小的测试.
- 适用于体外单细胞净化装置的运行.
主要成果:
- 噪音很大,并行BO显著超过了行业最先进的.
- 与现有方法相比,该方法需要平均减少约50%的实验.
- 在体外单细胞净化过程中成功应用,证明了其实用性.
结论:
- 噪音,并行贝叶斯优化是优化复杂生物过程的强大工具.
- 这种方法在细胞治疗过程开发中提供了实质性的效率提升.
- 这些发现支持在工业生物处理中采用先进的BO技术.
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