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外聚糖的结构性特征和功能性研究由本地Lacticaseibacillus rhamnosus P14从摩洛哥地区分离出来
Akhtach Sihame1, Tabia Zakaria2, Mabrouk El Khalil3
1Laboratory of Biotechnology, Environment, Agrifood, and Health (LBEAS), Faculty of Science Dhar Mahraz, University Sidi Mohamed Ben Abdallah, P.B 1796, Atlas Fez, Morocco.
Current microbiology
|February 19, 2024
概括
来自Lacticaseibacillus rhamnosus P14的新型外聚糖 (EPS) 具有显著的抗氧化和乳化特性. 这种生物活性多糖因其独特的结构和稳定性而有望用于各种应用.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 聚合物科学 聚合物科学
背景情况:
- 外聚糖 (EPS) 是微生物聚合物,具有多样化的结构和生物活性.
- 乳酸细菌 (LAB) 是已知的结构多样化的EPS的生产者.
- 了解EPS的物理化学和生物特性对于其应用至关重要.
研究的目的:
- 为了描述一种来自野生Lacticaseibacillus rhamnosus菌株的新型外聚糖化物 (EPS-P14).
- 为了研究EPS-P14的物理化学特性.
- 评估EPS-P14.的生物活性,包括抗氧化和乳化能力.
主要方法:
- 净化和表征EPS-P14. 的特性.
- 使用FT-IR,NMR,XRD,TGA,DSC和SEM进行物理化学分析.
- 评估抗氧化活性 (DPPH清除,铁的减少) 和乳化特性.
主要成果:
- EPS-P14是一种多孔,热稳定的 (在307°C降解) 均的α-D-glucan.
- 核磁共振和FT-IR证实主要是α-(1→6) 糖化链与α-(1→3) 分支.
- EPS-P14表现出高水溶性,度依赖的乳化和稳定作用,以及显著的抗氧化能力.
结论:
- EPS-P14具有独特的物理化学特性和显著的生物活性.
- 描述的外聚糖是食品,制药或生物技术应用的有希望的候选物.
- 根据其生物活性概况,对EPS-P14应用的进一步研究是有必要的.
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