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将UCST引入酸盐,以实现简单有效的单相提取

Thanit Kertsomboon¹, Chahya Kreangkaiwal², Kanitha Patarakul²

¹Center of Excellence in Bioresources to Advanced Materials (B2A-CE), The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand.

Biomacromolecules

|February 19, 2024

概括

基酸基聚合物表现出高临界溶液温度 (UCST) 行为,使得热响应功能. 这项研究表明它们在抗体结合系统中用于有效的抗原提取.

科学领域:

生物材料科学生物材料科学
聚合物化学聚合物化学
免疫技术是一种免疫技术.

背景情况:

高临界溶液温度 (UCST) 聚合物为控制释放和分离等应用提供热反应性质.
将UCST单体与功能化乙烯单体的共聚化可以引入新的特性,但UCST性能和生物相互作用需要仔细考虑.
奇托 (CS) 是一种天然的多糖,作为一种多功能生物聚合物骨干,用于结合.

研究的目的:

开发一种基于奇托的新型热敏聚合物系统,用于抗原提取.
将UCST聚合物和抗体结合到基托骨干上,以进行选择性结合和分离.
为了证明使用基托基UCST聚合物用于单相抗原提取的可行性.

主要方法:

使用酸作为链传递剂的UCST聚甲胺 (PMAAm) 的基性聚合.
通过N-糖胺 (N-Hydroxysuccinimide,NHS) 复合物对酸盐进行水基改性,从而产生CS-PMAAm.
对抗LipL32单克隆抗体 (mAb) 与CS-PMAAm的结合,以产生CS-PMAAm-Ab用于特定抗原结合.

主要成果:

合成的CS-PMAAm表现出UCST行为,云点在10-30°C之间.
抗体结合的CS-PMAAm-Ab具有选择性结合的重组LipL32 (rLipL32) 抗原 (Ag).
CS-PMAAm-Ab-Ag的聚合促进了从溶液中提取rLipL32.

结论:

素可以被功能化以表达UCST特性,从而产生热敏生物材料.
对CS-PMAAm的抗体结合使得特定抗原的识别和结合成为可能.
这种方法提供了一种简单而有效的方法,用于单相抗原提取,使用热响应性,抗体结合的奇托聚合物.

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