胆红素向下调节氧化应激和纤维细胞生长因子23在UMR106骨质细胞样细胞中的表达
Steffen Rausch1, Katharina Hammerschmidt1, Martina Feger1
1University of Hohenheim, Department of Physiology, Stuttgart, Germany.
概括
胆红素通过降低氧化应激,减少骨细胞中纤维细胞生长因子23 (FGF23) 的产生. 这一发现表明比利鲁宾.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 内分泌学 在内分泌学.
背景情况:
- 纤维细胞生长因子23 (FGF23) 调节酸盐和维生素D的代谢.
- 升高的FGF23与脏和心血管疾病的不良结果有关.
- 骨细胞产生的FGF23受氧化应激的影响.
研究的目的:
- 为了研究 bilirubin 对 FGF23 生产的影响.
- 探索氧化应激在比利鲁宾FGF23.23调节中的作用.
主要方法:
- 实验中使用了UMR106骨质母细胞样细胞.
- 通过定量实时PCR测量FGF23基因转录.
- 使用ELISA和CellROX测定分别评估了FGF23蛋白水平和细胞氧化应激.
主要成果:
- 未结合和结合的白素降低了FGF23基因转录.
- 胆红素治疗导致FGF23蛋白水平降低.
- 细胞氧化应激水平被 bilirubin 减少.
结论:
- 胆红素降低了骨质细胞样细胞中FGF23的产生.
- 胆红素减少FGF23的产生,很可能是通过减少细胞氧化应激作用.
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