PbrWRKY62-PbrADC1模块通过调节putrescine生物合成,涉及到Pyrus bretschneideri Rehd.果的表面发育
Xu Zhang1, Lijuan Zhu2, Ming Qian1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Molecular horticulture
|February 19, 2024
概括
梨水果表面的皮与putrescine有关. 研究人员确定了PbrWRKY62-PbrADC1通路,这对于素生物合成至关重要,并提高了水果冷却耐受性.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 表面烧伤是冷藏期间梨子果的主要收获后疾病.
- 普特雷辛的积累与表面烧伤的发展有关,但根本的分子机制尚未完全理解.
研究的目的:
- 为了阐明子生物合成影响表面烧焦的发展和梨果的冷却抵抗的分子机制 (P. bretschneideri Rehd. ) 的情况.
主要方法:
- 在普特雷辛代谢途径中对代谢和转录基因特征的联合分析.
- 对基因功能的实验验证,包括蛋白质二分化和酶活性测试.
- 对转录因子结合部位和基因表达调节的分析.
主要成果:
- PbrADC1是叶绿体中的同型蛋白质,参与了素的生物合成,并增强了水果的冷却抵抗力.
- 在PbrADC1中的Cys546残留物对阿金脱碳化至关重要,其活性由H2O2调节.
- 转录因子PbrWRKY62直接与PbrADC1促进体结合,激活其表达并增加雷素水平.
结论:
- 该PbrWRKY62-PbrADC1模块调节了putrescine生物合成,影响了梨果的表面烧焦发展和冷却耐受性.
- 这些发现为培育具有增强抗表面烧伤能力的梨提供了宝贵的遗传见解.
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