相关实验视频

Updated: Jul 2, 2025

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Published on: May 12, 2017

调节人类寡细胞中压力颗粒的形成

Florian Pernin¹, Qiao-Ling Cui¹, Abdulshakour Mohammadnia¹

¹Neuroimmunology Unit, Montreal Neurological Institute, McGill University, Montreal, QC, Canada.

Nature communications

|February 19, 2024

概括

在多发性硬化症 (MS) 病变中,压力颗粒 (SG) 在寡类细胞 (OLs) 中形成. 它们的持久性表明细胞对大脑中代谢和炎症压力的联合反应.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
病理学病理学病理学

背景情况:

寡头质细胞 (OL) 的损伤和损失是多发性硬化症 (MS) 的关键特征.
压力颗粒 (SG) 是参与应激反应的细胞结构,隔离停滞的mRNAs.
在MS病理学的背景下,SG在OL中的作用尚不清楚.

研究的目的:

调查多发性硬化症 (MS) 中的寡细胞 (OLs) 中压力颗粒 (SGs) 的存在和形成.
确定诱导SG形成和人类OLs持久性的条件.
阐明OLs中代谢压力,炎症和SG动态之间的关系.

主要方法:

对多发性硬化病变和正常外观白质的免疫组织化学分析,用于OLs中的SG标记物.
在体外培养初级人类成年人大脑衍生OLs.
诱导压力状况,包括代谢性压力,促炎性细胞因子和糖溶性抑制.
使用显微镜和生化分析评估SG形成和持久性.

主要成果:

在活跃和不活跃的多发性硬化病变中,以及在看起来正常的白质中,观察到压力颗粒 (SG) 在寡基细胞 (OL) 中.
代谢应激诱导过渡性SG形成在培养的人类OLs.
将代谢压力与促炎性细胞因子结合起来导致持久性SG,与仅由代谢压力引起的短暂SG不同.
持续的SG形成与糖溶性抑制有关,突出了对细胞能量特性的依赖.

结论:

在多发性硬化症 (MS) 中,压力颗粒 (SG) 在寡类细胞 (OL) 中的持久性反映了细胞对代谢压力和促炎症条件的组合的反应.
这些发现表明,代谢失调和炎症有助于MS中OLs中的SG积累.
了解OL中的SG动态可能会为MS的发病和潜在的治疗点提供洞察力.

更多相关视频

Visualization of G3BP Stress Granules Dynamics in Live Primary Cells

Visualization of G3BP Stress Granules Dynamics in Live Primary Cells

Published on: May 21, 2014

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Published on: November 9, 2020

相关概念视频

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...