TREX揭示了与活细胞中特定RNA区域结合的蛋白质
Martin Dodel1, Giulia Guiducci1, Maria Dermit1
1Centre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Nature methods
|February 19, 2024
概括
研究人员开发了TREX (有针对性的RNase H介导的交联RBP的提取),这是一种识别与活细胞中特定RNA区域相互作用的RNA结合蛋白的新方法. 该工具以位置和公正的方式绘制RNA-蛋白相互作用的地图.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生化学
背景情况:
- RNA结合蛋白 (RBPs) 通过与特定RNA区域的相互作用来调节基因表达.
- 目前的方法缺乏在内源条件下与活细胞中的特定RNA区域相互作用的RBP无偏的识别能力.
研究的目的:
- 引入一种新的,敏感的方法来识别活细胞中与特定RNA区域结合的RBP.
- 为了使内源性RNA-蛋白相互作用的无偏见,定位映射.
主要方法:
- TREX (有针对性的RNase H介导的交联RBPs提取) 使用有针对性的RNase H活动来隔离与特定RNA区域结合的交联RBPs.
- 该方法应用于U1 snRNA,NORAD长非编码RNA和45S rRNA.
主要成果:
- 与现有方法相比,TREX在识别已知的U1 snRNA交互体方面表现出卓越的性能.
- 该研究揭示了NORAD长非编码RNA的新型,区域特异的内源相互作用体.
- 创建了一个全面的45S rRNA区域间互动组,识别了对核糖体生物发生至关重要的已知和新相互作用.
结论:
- TREX是一种高度敏感的,以RNA为中心的工具,用于对活细胞内源性RNA-蛋白相互作用的无偏的位置映射.
- 这种方法通过精确识别RBP结合位点,提高了对RNA调节和功能的理解.
- TREX适用于不同的细胞类型,在分子生物学研究中具有广泛的实用性.
更多相关视频
10:52Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Published on: September 28, 2017
8.1K
08:46Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
Published on: May 18, 2020
5.9K
相关概念视频
02:43Nucleic Acids
44.2K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
44.2K
01:23Types of RNA
63.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.7K
02:36Transfer RNA Synthesis
11.9K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
11.9K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
01:19RNA Structure
4.8K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
4.8K
01:23Regulation of Expression at Multiple Steps
907
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
907