来自印度的Chromobacterium piscinae的部分基因组表征揭示了多种药物耐药性
Binod Kumar Choudhary1, Mamta Choudhary2, Sukhadeo B Barbuddhe3
1Indian Council of Agricultural Research-National Institute of Biotic Stress Management, Baronda, Raipur, 493 225, Chhattisgarh, India. binods14@gmail.com.
概括
这项研究从社区池中分离和测序了Chromobacterium piscinae,揭示了抗生素耐药性基因和潜在的公共卫生风险. 这种基因组分析突出显示了细菌的细菌.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 克罗莫细菌 (Chromobacterium) 属因其代谢灵活性和致病潜力而闻名.
- 在全球范围内已经分离出了多种染色体细菌物种,这引发了各种生物技术和健康方面的担忧.
研究的目的:
- 从一个社区池中分离和描述Chromobacterium piscinae.
- 执行基因组组装草案和对隔离物的详细序列分析.
- 评估其抗生素耐药性和毒性的基因组基础.
主要方法:
- 细菌的分离和生物化学表征.
- 矩阵辅助激光吸附/离子化飞行质谱时间 (MALDI TOF-MS).
- 使用Illumina HiSeq 4000进行部分基因组测序,并使用PATRIC进行综合分析.
主要成果:
- 成功隔离和测序了Chromobacterium piscinae菌株W1B-CG-NIBSM. 这种菌株是什么?
- 基因组草案 (4.16 Mb, 62.69% GC含量) 包含4,126个蛋白质编码序列 (CDS).
- 鉴定了与抗生素耐药性 (45),药物点 (6),载体 (3) 和毒性因子 (10) 相关的基因,赋予了多种药物耐药性,特别是对β-乳糖胺.
结论:
- 基因组分析揭示了Chromobacterium piscinae的适应性,代谢途径和毒性因素.
- 已证实该分离物的多药耐药性,特别是对β-乳酸盐的耐药性.
- 从社区池中隔离突出了潜在的公共卫生危险,代表了印度C. piscinae的第一个基因组描述.
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