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Dorna Rostamnejad¹, Fatemeh Esnaashari², Hossein Zahmatkesh¹

¹Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University (IAU), Lahijan, Guilan, Iran.

Archives of microbiology

|February 20, 2024

概括

迪克洛菲纳克含的多分子 (乳酸与甘油酸) 纳米颗粒 (DS-PLGA NPs) 有效地抑制了Pseudomonas aeruginosa的毒性. 这些纳米粒子减少生物膜的形成和定数感应基因表达,为治疗P. aeruginosa感染提供了有前途的方法.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
微生物学微生物学

背景情况:

聚乳合甘油酸 (PLGA) 是一种生物相容的聚合物,以可控药物释放而闻名.
由于它们的毒性因素,Pseudomonas aeruginosa感染带来了重大挑战.

研究的目的:

为了合成和表征二甲加载PLGA纳米粒子 (DS-PLGA NPs).
评估DS-PLGANP对P. aeruginosa的抗菌和抗病毒潜力.
调查DS-PLGANP对P. aeruginosa.的定数感应 (QS) 基因表达的影响.

主要方法:

使用溶剂蒸发技术准备了DS-PLGA NP.
纳米粒子表征涉及SEM,DLS和泽塔潜力分析.
抗菌和抗病毒性测试包括汁微稀释,水晶紫色染色,血液溶解和抽运动性测试.
实时PCR用于评估QS基因表达 (lasI和lasR).

主要成果:

合成的NP是球形 (60-150nm) 的,其泽塔电位为-15.2mV.
针对P. aeruginosa的DS-PLGANP的MIC在4.5至9毫克/毫升之间.
DS-PLGA NPs显著下调了lasI和lasR基因表达 (0.51和0.75倍).
NP降低了生物膜形成 (9-27%),血液溶解活性 (32-88%) 和动力 (0-32.3%).

结论:

DS-PLGA NPs显示出对P. aeruginosa.具有显著的抗病毒活性.
突出了DS-PLGA NP的反定数感应特性.
这些纳米颗粒代表了一种潜在的安全和有效的策略,用于管理P. aeruginosa感染.

关键词:

生物膜是一种生物膜.狄克洛芬雅克的使用方法在 PLGA PLGA 中.议员数量检测是指对议员数量进行检测.

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