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IGFBP1a是一种营养缺乏反应因子,可以通过下丘脑-垂体-卵巢轴†抑制鱼类的繁殖

Wei Li¹, Hangyu Li¹, Qiongyao Hu¹

¹Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan, China.

Biology of reproduction

|February 20, 2024

概括

禁食通过增加胰岛素类生长因子结合蛋白1 (IGFBP1) 来抑制鱼类的繁殖. 这种蛋白质会破坏下丘脑-垂体-阴茎轴,阻碍阴茎发育和生殖基因表达.

科学领域:

内分泌学在内分泌学.
生殖生物学生殖生物学
鱼类生理学鱼类生理学

背景情况:

生殖需要大量的能量,营养不良/禁食会损害生殖腺发育.
禁食抑制繁殖的精确分子机制在很大程度上是未知的.

研究的目的:

为了研究在草中禁食诱导的繁殖抑制背后的分子机制.
确定参与禁食反应影响生殖系统的关键分子参与者.

主要方法:

在禁食条件下对草在各种组织 (肝脏,下丘脑,垂体,卵巢) 中的胰岛素类生长因子结合蛋白1 (IGFBP1) 表达的定量分析.
试验室研究评估IGFBP1a对下丘脑细胞,垂体细胞和卵巢细胞基因表达的影响.
关键生殖基因的分析,包括淋巴激素释放激素3 (GnRH3),黄素化激素 (LH),毛囊刺激激素 (FSH) 和类固醇酶.

主要成果:

禁食显著上调IGFBP1表达在肝脏,下丘脑,垂体和卵巢的草.
发现IGFBP1a通过作用于下丘脑-垂体-阴茎轴来抑制阴茎的发育.
IGFBP1a抑制了下丘脑中的GnRH3表达,通过干扰类似胰岛素的生长因子1信号传递,降低了垂体中的LH和FSH表达,并直接抑制了卵巢受体 (lhr,fshr) 和类固醇基因 (cyp11a,cyp17a,cyp19a1) 的表达.

结论:

禁食诱导IGFBP1的表达,它作为一个营养缺乏反应因子.
IGFBP1a通过在多个层面上扰乱垂体-垂体-卵巢轴来抑制鱼类的繁殖,从中央信号到卵巢功能.

关键词:

在 IGFBP1 中.禁食禁食是一種禁食.在下丘脑中,下丘脑卵巢卵巢是一个卵巢.pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary pituitary 下垂体下垂体下垂体下垂体下垂体下垂体下垂体下垂体生殖生殖的繁殖.

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