设计和验证一套定制的系统,用于测量在活跃存储机器中保存的人类角膜的晶状体电阻
Marielle Mentek1, Benjamin Peyret1, Siwar Zouari1
1Laboratory of Biology, Engineering and Imaging for Ophthalmology (BiiO), EA2521, Faculté de Médecine, Université de Jean Monnet, 10 rue de la Marandière, 42270 Saint-Etienne, France.
International journal of pharmaceutics: X
|February 20, 2024
概括
一个新的系统非侵入性地测量角膜的电特性ex-vivo. 在主动存储机器 (ASM) 中存储的人类角膜显示恢复了屏障功能,支持它们作为药物输送模型的使用.
科学领域:
- 眼科医生 眼科 眼科
- 生物医学工程 生物医学工程
- 药理学 药理学是指药理学的学科.
背景情况:
- 角膜上皮屏障是眼部药物输送中的一个关键挑战.
- 评估电气性能提供了一种非侵入性的方法来评估屏障功能.
- 在活性存储机 (ASM) 中保存的人类角膜是药物透研究的潜在模型.
研究的目的:
- 开发和验证一种新的ex-vivo系统来评估角膜上皮质屏障功能.
- 评估存储在ASM中的人类角膜的电特性.
- 为了确定ASM保存的人类角膜作为药理模型的适用性.
主要方法:
- 设计了一个带有Ag/AgCl电极和专用软件的双极系统.
- 该系统在带有表皮缺陷的猪角膜和暴露于化 (BAC) 的猪身上进行了测试.
- 该系统应用于在ASM中存储14天的人类角膜.
主要成果:
- 猪角膜显示,随着的大小和BAC暴露的增加,阻力下降.
- 在ASM中存储的人类角膜呈现出增加的角膜阻抗.
- 通过ASM保存的角膜显示恢复了多层表皮和增强了紧结标记表达 (zonula occludens 1, claudin 1, occludin).
结论:
- 开发的系统有效地评估角膜上皮质屏障功能ex-vivo.
- 在ASM中存储的人类角膜表现出改善的屏障完整性和功能.
- 通过ASM保存的人类角膜是眼部药物输送研究的一个有希望的生理模型.
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