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Updated: Jul 2, 2025

Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
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Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides

Published on: July 26, 2018

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通过结构导向工程对糖系转移酶UGT94D1的选择性合成rebaudioside M2

Lifeng Yang1,2, Mengliang Yang3, Zhiwei Deng3

  • 1School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, China.

Frontiers in bioengineering and biotechnology
|February 20, 2024
PubMed
概括

研究人员设计了一种新的酶变体,UGT94D1-F119I/D188P,以有效地产生Rebaudioside M2 (Reb M2). 这一突破提高了Reb M2的产量和选择性,为其工业应用铺平了道路.

关键词:
美国人 美国人 UGT94D1葡萄糖转移酶的使用方法蛋白质工程工程 蛋白质工程rebaudioside M2 的使用方法这是一种醇糖化物,steviol glycoside.

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相关概念视频

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
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