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来自Scoparia dulcis的植物可以在巨分化过程中防止NF-κB介导的炎症反应

Parimalanandhini Duraisamy¹, Annapoorani Angusamy¹, Sangeetha Ravi¹

¹Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025 India.

|February 20, 2024

概括

来自Scoparia dulcis的植物通过减少氧化应激和降低血标志物的调节来防止炎症性巨细胞分化. 这种天然化合物在治疗炎症性疾病方面表现有前途.

科学领域:

免疫学和药理学
自然产品化学自然产品化学

背景情况:

巨细胞通过它们的两极分化在炎症性疾病中发挥着关键作用.
巨细胞两极分化是治疗性干预炎症条件的关键目标.

研究的目的:

研究从Scoparia dulcis中分离出来的植物醇对巨细胞两极分化的免疫调节作用.
评估植物在预防M1巨分化和相关炎症反应方面的潜力.

主要方法:

植物醇从Scoparia dulcis中分离出来,并使用GC-MS,TLC,HPTLC,FTIR,1H-NMR和HPLC进行了表征.
通过使用7-基托胆固醇和细菌脂多糖化物,诱导IC-21细胞进行两极分化.
试验包括氧化应激,酶活性,膜潜能,和脂质调节,以及炎症和巨标记物的基因/蛋白质表达分析.

主要成果:

植物醇 (400μg/ml) 减少了氧化和反应性氧物种 (ROS) 的产生.
植物醇降低了和脂质积累,稳定了细胞膜,并增加了酶活性.
在基因和蛋白质水平上,植物降低了动脉动脉标记物 (iNOS,COX-2,IL-6,IL-1β,MMP-9,CD36,NF-κB) 和上调的巨细胞标记物 (CD206,CD163,PPAR-γ).

结论:

从Scoparia dulcis中分离出来的植物醇有效地阻止了M1巨细胞的两极分化.
植物具有显著的免疫调节和抗炎性质.
植物醇是一种有前途的天然化合物,用于控制炎症性疾病.

关键词:

这是一种免疫调节剂.植物醇 (Phytol) 是一种植物成分.两极化的偏化

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