波普鲁斯MYC2调节了防御通路的根转录重编程,以损害拉卡利亚双色球ectomycorrhizal的发展
José Eduardo Marqués-Gálvez1, Gaurav Pandharikar1, Veronica Basso1
1Université de Lorraine, INRAE, UMR 1136 Interactions Arbres-Microorganismes, Centre INRAE Grand Est-Nancy, Champenoux, 54280, France.
The New phytologist
|February 20, 2024
概括
斯酸通路调节了生体外生菌的共生. 木MYC2转录因子控制真菌殖民,过度表达减少,沉默增加,揭示了植物-真菌相互作用中的作用.
科学领域:
- 植物与微生物的相互作用
- 分子植物-真菌共生生态.
- 植物信号通道的信号通道.
背景情况:
- 素酸 (JA) 信号通路对于生殖内菌 (ECM) 协生至关重要.
- 拉卡利亚双色效应剂MiSSP7通过稳定JAZ6,抑制Populus MYC2转录因子来影响这种途径.
- MYC2在植物防御中的作用已知,但其在ECM共生中的特定功能需要阐明.
研究的目的:
- 为了调查Populus MYC2转录因子在生体外共生建立中的作用.
- 了解平衡植物免疫力和共生关系的分子机制.
- 为了确定参与ECM共生的MYC2调节基因.
主要方法:
- 转基因树的产生与改变的MYC2表达 (过度表达和沉默).
- 在转基因树中评估ectomycorrhizal殖民的评估.
- 转录组分析和DNA亲和力净化测序.
主要成果:
- 过度表达MYC2s减少了真菌殖民,而沉默增加了它.
- MYC2s直接或间接调节对ECM建立至关重要的基因.
- 合成酶基因的丰富表明宿主单在调节真菌生长中的作用.
结论:
- MYC2 转录因子和烯是根真菌在相互共生中的殖民性的重要调节者.
- MYC2在控制工厂防御和ECM建立之间的平衡方面发挥着至关重要的作用.
- 这项研究确定了参与MYC2-介导的ECM共生控制的关键基因.
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