相关实验视频
Updated: Jul 2, 2025

08:55
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
Published on: March 17, 2018
22.0K
斯基桑德林A通过抑制表皮生长因子受体激活,诱导非小细胞肺癌的亡
Linhai Zhu1, Yanye Wang1, Xuhua Huang1
1Department of Thoracic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Cancer medicine
|February 20, 2024
概括
斯基桑德林A (SchA) 通过抑制EGFR酸化,诱导非小细胞肺癌 (NSCLC) 亡. 这种机制涉及ERBB家族目标,可以通过激活EGFR绕道来抵消.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 非小细胞肺癌 (NSCLC) 仍然是癌症死亡的主要原因.
- 石素A (SchA) 是一种具有潜在抗癌特性的化合物.
- 了解SchaA在NSCLC中的精确分子机制对于治疗开发至关重要.
研究的目的:
- 阐明斯基桑德林A (SchA) 诱导非小细胞肺癌 (NSCLC) 亡的生物机制.
- 为了识别NSCLC细胞中受到SchA影响的分子标和信号通路.
主要方法:
- 使用瑞士目标预测,字符串数据库,基因本体学和基因和基因组的京都百科全书的网络药理方法.
- 分子对接和化学蛋白相互作用学,以预测和验证SchA-目标相互作用.
- 通过Western blot,Annexin V/propidium iodide染色和细胞周期分析进行实验验证.
主要成果:
- 预计SchA的目标是酶,ERBB家族成员和SRC被确定为蛋白质-蛋白质相互作用网络中的中心节点.
- 发现SchA与EGFR氨酸激酶域的ATP活性囊绑定,抑制其酸化.
- SchA诱导的细胞毒性被胰岛素部分抵消,对EGFR过度表达或EGF/IGF-1过量的影响有限.
结论:
- 网络药理学表明,ERBB家族成员是NSCLC中SchA的关键目标.
- SchA主要通过抑制EGFR酸化来抑制NSCLC的亡.
- EGFR旁路激活可以中和SchA的细胞毒性作用,突出显示潜在的治疗脆弱性.
相关概念视频
Mitogens and the Cell Cycle
6.5K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K
The Intrinsic Apoptotic Pathway
6.5K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.5K
The Extrinsic Apoptotic Pathway
6.4K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K

