在白色腐烂木材腐烂过程中,碳水化合物活性酶的甲酸氧化
Lise Molinelli1,2, Elodie Drula1,3, Jean-Charles Gaillard4
11Biodiversité et Biotechnologie Fongiques, INRAE, Aix Marseille Université, Marseille, France.
Applied and environmental microbiology
|February 20, 2024
概括
白色腐烂真菌通过降低甲胺含量和使用催化酶来保护分泌的酶免受氧化. 这项研究确定了碳水化合物活性酶中的氧化残留物,这些酶对基纤维素降解至关重要.
科学领域:
- 生物化学 生化学
- 菌类学 菌类学是指菌类学.
- 蛋白质组学是指蛋白质组学.
背景情况:
- 白色腐烂真菌通过分泌的碳水化合物活性酶 (CAZymes) 和反应性氧物种 (ROS),如过氧化 (H2O2) 降解纤维素.
- ROS对分泌蛋白质氧化,特别是氨酸 (Met) 残留物的影响尚不清楚.
- 甲氧化成甲硫氧化物或甲硫可以产生各种功能后果.
研究的目的:
- 为了研究白色腐烂真菌Pycnoporus cinnabarinus*中分泌的蛋白质,特别是 metionin残留物的氧化.
- 识别氧化蛋白和残留物,并了解防护机制,防止ROS诱导的损害.
- 确定catalase在保护分泌蛋白质免受氧化中的作用.
主要方法:
- 在树木上种植Pycnoporus cinnabarinus.
- 在分泌的蛋白质中分析氨酸含量,使用深度猎枪蛋白质组学与H218O2标记相结合.
- 在分泌的蛋白质中识别氧化氨酸和氨酸残留物.
主要成果:
- 从*P. cinnabarinus*分泌的蛋白质具有明显较低的氨酸含量 (1.4%),与细胞内蛋白质 (2.1%) 相比.
- 一种分泌的催化酶有效地保护蛋白质免受氧化.
- 在分泌的蛋白质中发现了49种可氧化氨酸和40种可氧化氨酸残留物,主要是CAZymes,一些Met残留物氧化高达47%.
结论:
- 白色腐烂真菌在分泌的蛋白质中最大限度地降低了氨酸含量,并利用催化酶减轻ROS损伤.
- 特定的氧化 metionin 残留物,特别是 GH7 纤维化水解酶,是保存的,并且可能在纤维化纤维素降解中发挥功能性作用.
- 这项研究揭示了真菌在木材腐烂期间保护蛋白质的适应策略,并突出了CAZymes中的氧化还原活性位.
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