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Updated: Jul 2, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

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Published on: September 7, 2022

通过针对核体蛋白质的ssDNA向体抑制SARS-CoV-2复制的抑制

Yanping Huang^1,2, Congcong Huang^1,2, Junkai Chen^1,2

¹Department of Infectious Diseases, Renmin Hospital, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China.

Microbiology spectrum

|February 20, 2024

概括

一种新型的DNA吸收体,cb-N-Apt17,有效地向SARS-CoV-2核囊蛋白. 这种阿普坦酶破坏了病毒液-液相分离,显示了新的COVID-19抗病毒疗法的潜力.

科学领域:

病毒学病毒学
分子生物学分子生物学
药物发现药物发现药物发现

背景情况:

SARS-CoV-2 核体 (N) 蛋白对于病毒组合,免疫逃避和稳定性至关重要.
跨菌株的N蛋白的保存使其成为COVID-19抗病毒疗法的有吸引力的目标.
在液-液相分离 (LLPS) 中N蛋白的作用对于病毒复制至关重要.

研究的目的:

为了识别和表征向SARS-CoV-2核囊蛋白的DNA吸附体.
为了评估一个修饰的aptamer,cb-N-Apt17,在破坏N蛋白LLPS.在破坏N蛋白的有效性.
评估cb-N-Apt17对抗COVID-19的治疗潜力.

主要方法:

单链DNA体N-Apt17的鉴定和特征.
设计和合成一个圆形双价阿普坦体,cb-N-Apt17.
在体外和体内对阿普他默稳定性,结合亲和力和抑制N蛋白LLPS的评估.

主要成果:

N-Apt17有效地破坏了SARS-CoV-2的N蛋白LLPS.
圆形双价形式,cb-N-Apt17,表现出增强的稳定性和结合亲和力.
cb-N-Apt17显示出N蛋白LLPS的优异抑制,这表明它有可能抑制病毒复制.

结论:

cb-N-Apt17是COVID-19的一个有前途的治疗候选药物.
用阿普坦酶准N蛋白LLPS为抗病毒开发提供了一个可行的策略.
进一步开发cb-N-Apt17可能会导致针对SARS-CoV-2变种的新型治疗方法.

关键词:

这就是SARS-CoV-2病毒.抗病毒疗法是一种抗病毒疗法.一个aptamer的应用程序.核体蛋白质是核体蛋白质的一种.

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