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从Mythimna separata中分离出来的新型alphabaculovirus的进化分析和生物特征

Tao Dou^1,2,3, Futao Gao^1,2,3, Junhua Zhu^1,2,3

¹College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, PR China.

The Journal of general virology

|February 20, 2024

概括

从Mythimna separata中分离出来的一种新型细菌病毒,Mamestra brassicae多重核聚合体病毒CHN1 (MbMNPV-CHN1),对七种白类动物有很高的传染性. 这种baculovirus提供了作为生物杀虫剂的潜力.

科学领域:

* 病毒学病毒学
* 昆虫学昆虫学
* 分子生物学 * 分子生物学

背景情况:

* 百科病毒是昆虫特有的病原体,具有生物害虫控制的潜力.
*对新型细菌病毒分离物的准确分类和理解对于它们的应用至关重要.
* 对新型细菌病毒分离物的研究提高了可持续害虫管理策略的选择.

研究的目的:

* 从Mythimna separata中分离和描述一种新型的细菌病毒.
* 确定完整的基因组序列并对新分离物进行分类.
* 评估baculovirus作为生物杀虫剂的传染性和潜力.

主要方法:

*从Mythimna separata幼虫中分离出百科病毒.
*使用下一代测序技术进行全基因组测序.
* 遗传学分析,基因组比较和超结构分析用于分类.
*针对各种Lepidoptera物种的传染性测定.

主要成果:

* 一种名为Mamestra brassicae多核多重合体病毒CHN1 (MbMNPV-CHN1) 的新型细菌病毒被分离出来,并对其基因组进行了测序 (153,882 bp,163 ORF).
* 遗传学分析将MbMNPV-CHN1置于Mb-McNPV组内,显示与相关病毒的高度对齐性.
* MbMNPV-CHN1对七种白类动物表现出高感染力,在M. separata幼虫中具有显著的OB产量.

结论:

* MbMNPV-CHN1是Mamestra brassicae多核多重体病毒的一个独特变体,有助于了解Mb-McNPV群.
*在Mb-McNPV组中,使用偏差主要受到自然选择的影响.
* MbMNPV-CHN1显示出作为一种特定且有效的生物杀虫剂来控制虫虫害的有希望的潜力.

关键词:

马姆斯特拉·布拉西卡多个核多重体病毒.进行比较的基因组学.主机范围的主机范围.传染性的感染性.新型的阿尔法巴库洛病毒.人类的基因组学.

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