米NLR蛋白XinN1,由模式识别受体XA21诱导,增强了对细菌病的抵抗力
Hyeran Moon1,2, A-Ram Jeong1, Chang-Jin Park3,4
1Department of Molecular Biology, Sejong University, Seoul, Republic of Korea.
Plant cell reports
|February 20, 2024
概括
米植物具有模式识别受体 (PRR) 和核酸结合域白丰富的重复含有受体 (NLR) 免疫. 一个新发现的NLR,XinN1,被PRR XA21激活,增强了对Xoo.引起的细菌病害的抗米能力.
科学领域:
- 植物免疫力 植物免疫力
- 植物病原体相互作用
- 分子遗传学 分子遗传学
背景情况:
- 植物利用模式识别受体 (PRRs) 进行模式触发免疫 (PTI) 和核酸结合域氨酸丰富的重复含有受体 (NLRs) 进行效应器触发免疫 (ETI).
- 大米PRR XA21可以识别来自Xanthomonas oryzae pv. 的. 疹 (Xoo),启动免疫反应.
- 了解PRRs和NLRs之间的相互作用对于增强作物抗病能力至关重要.
研究的目的:
- 为了识别和表征一种新的米NLR基因,该基因参与XA21介导的免疫力,对Xoo.
- 调查新N1在植物防御途径中的作用及其对疾病耐药性的贡献.
主要方法:
- 米的基因鉴定和克隆NLR XinN1.1.
- 生产转基因大米植物,过度表达XinN1及其自身活性突变体.
- 对抗疾病,病变长度和细菌生长的表型分析.
- 测量反应性氧物种 (ROS) 生产和在米原生质中无细胞质 (Ca2+) 的积累.
- 对PTI信号组件基因表达的分析.
主要成果:
- 在XA21介导的对Xoo.Xoo的免疫过程中,XinN1被特别诱导.
- 过度表达XinN1及其自身活性突变体增强了大米对Xoo的抗性.
- 新N1过度表达导致ROS产量增加和Ca2+积累.
- 新N1诱导了PTI信号组件的转录,包括OsSERK和OsRLCK.
结论:
- 米CC型NLR XinN1,由PRR XA21诱导,在激活对Xoo的防御途径方面发挥着重要作用.
- 新N1有助于提高大米的抗病能力,可能通过ROS和Ca2+信号传递.
- 这项研究阐明了大米免疫系统的关键组成部分,为开发抗病大米品种提供了洞察力.
相关概念视频
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Types of RNA
63.7K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.7K
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K


