在由Mucor circinelloides发酵的猪背脂中产生多不和脂肪酸
Haisu Shi1,2,3, Yingtong Zhang4, Hao Lin1
1College of Food Science, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
Applied microbiology and biotechnology
|February 20, 2024
概括
这项研究转换了猪背部脂肪.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 猪背脂 (PB) 富含和脂肪酸 (SFAs),但缺乏多不和脂肪酸 (PUFAs).
- 微生物将SFAs转化为PUFA和单不和脂肪酸 (MUFA) 可以提高PB的营养价值.
- 乳腺菌和乳腺菌 (Lactiplantacillus plantarum) 是适合这种生物转换的微生物.
研究的目的:
- 开发一种使用共培微生物将PB中的SFAs转化为PUFA的方法.
- 优化发酵条件,以提高PB中的PUFA生产.
- 研究促进PB的内部层内的微生物生长的技术.
主要方法:
- 共同培养的 Mucor circinelloides CBS 277.49 和 Lactiplantacillus plantarum CGMCC 24189 用于 PB 发酵.
- 使用超声波和帕帕因来促进M.circinelloides透到内部PB层.
- 调整氧气和通风率以优化内部微生物生长.
- 在18°C发酵PB以最大限度地提高PUFA产量.
主要成果:
- 发酵猪背脂 (FPB) 达到显著水平的玛-烯酸 (GLA) 和烯酸 (LA).
- 超声波和帕帕因治疗增加了第三层脂肪层的GLA,达到2.58 mg/g.
- 优化氧气 (2米/秒) 和通风 (18米3/小时) 将第三层的GLA增加到4.13毫克/克.
- 18°C的发酵产生了高GLA (15.73 mg/g表面) 和LA (105.45 mg/g第二层,98.46 mg/g第三层).
结论:
- 在PB中过剩的SFAs可以有效地转化为有价值的PUFAs.
- 共同培养M. circinelloides和L. plantarum提供了一种可行的方法来丰富PB的PUFA.
- 超声波,帕帕因和受控通风是增强PB内微生物活动和PUFA生产的关键技术.
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