评估Bacillus分离物作为对土壤中传播的植物病原菌的生物控制剂
Derya Maral-Gül1, Rengin Eltem2
1Graduate School of Natural and Applied Sciences, Department of Bioengineering, Ege University, 35100, Bornova-Izmir, Türkiye. deryamaralgul@gmail.com.
概括
研究人员确定了19种具有强烈抗真菌性能的非致病性Bacillus subtilis菌株. 这些Bacillus菌株显示出开发生物控制产品,促进可持续农业和减少农药使用的潜力.
科学领域:
- 农业微生物学 农业微生物学
- 植物病理学 植物病理学
- 生物技术是生物技术.
背景情况:
- 在农业中使用农药会给环境和人类健康带来风险.
- 使用微生物进行生物控制为化学农药提供了一个可持续的替代方案.
- 细菌物种以其作为生物控制剂的潜力而闻名.
研究的目的:
- 识别和描述具有抗真菌活性的细菌分离物对关键植物病原体的抗真菌活性.
- 评估选择的细菌菌株的生物控制潜力.
- 评估农业应用中有前途的分离物的安全性.
主要方法:
- 对1574种Bacillus分离物的查,以检测它们对Botrytis cinerea,Fusarium solani和Rhizoctonia solani的抗真菌活性.
- 评估酶活动 (蛋白酶,基酶,基酶) 和抗真菌代谢产物 (酸,酸).
- 用16S rRNA基因测序评估凝结酶活性的致病性和分子识别.
主要成果:
- 在1574个分离物中,有77个呈现出针对所有测试病原体的抑制区.
- 19种非致病性Bacillus分离物表现出显著的抗真菌作用,并产生有益的酶和代谢物.
- 分子鉴定证实了这些19个分离物是Bacillus subtilis菌株.
结论:
- 原生Bacillus subtilis菌株在体外具有强大的抗真菌能力.
- 这些选择的菌株是非致病的,适合用于生物控制应用.
- 鉴定到的Bacillus菌株为开发用于可持续农业的新型生物控制产品提供了有希望的基础.
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