酶氧化方法与用火焰电离探测器进行头部气体染色学之间的比较,以确定死后血液中乙醇的含量
Nguyen Thi Thanh Xuan1, Dinh Vu Le2, Mai Thi Thanh1
1Forensic Medicine Center of Ho Chi Minh City, Ho Chi Minh City, 700000, Vietnam.
Forensic science, medicine, and pathology
|February 20, 2024
概括
在死后血液中乳酸和乳酸脱酶 (LDH) 的升高会干扰使用酶氧化方法测量血中酒精度 (BAC). 用火焰电离探测器 (HS-GC / FID) 进行头部气体染色学,为法医毒理学提供了更准确的替代方案.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 血液中酒精度 (BAC) 在法医毒理学中至关重要,酶氧化是一种常见的方法.
- 尸体样本经常显示乳酸和乳酸脱酶 (LDH) 水平升高,这可能会影响BAC的准确性.
- 由于死后的生物变化,现有的方法可能会产生不准确的BAC结果.
研究的目的:
- 调查乳酸和LDH对死后血液中BAC测定的影响.
- 为了比较酶氧化方法的准确性与头空间气色谱-火焰电离探测器 (HS-GC/FID).
- 在法医解剖中推一种可靠的BAC评估方法.
主要方法:
- 分析了110个尸体解剖的血液样本.
- 测量乳酸盐和LDH水平.
- 使用酶氧化和HS-GC/FID方法确定BAC.
- 统计分析以评估相关性和意义.
主要成果:
- 尸体血样显示乳酸和LDH水平高于正常血液.
- 在LDH水平和BAC差异之间发现了显著的正相关性 (r=0.67,p<0.001).
- 酶氧化方法显示显著干扰,导致乳酸和LDH升高时在BAC确定中出现正误.
结论:
- 死亡后血液中乳酸和LDH水平的升高显著干扰了通过酶氧化方法的BAC测定.
- HS-GC/FID是一种更准确的方法,用于在死后样本中进行BAC评估.
- 在法医诊断中准确的BAC确定需要使用HS-GC/FID,而不是死后样本的酶方法.
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