在老年COVID-19患者中,炎症媒介与线粒体DNA变异的关联
Tiziana Casoli1, Anna Rita Bonfigli2, Mirko Di Rosa3
1Center for Neurobiology of Aging, IRCCS INRCA, Ancona, Italy.
Aging and disease
|February 20, 2024
概括
老年COVID-19患者表现出线粒体DNA (mtDNA) 突变的增加,特别是呼吸复合体I基因中的低水平异质体. 这些mtDNA变化与较高水平的关键炎症生物标志物相关,这表明SARS-CoV-2感染和线粒体损伤之间存在联系.
科学领域:
- 线粒体生物学 线粒体生物学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- COVID-19对老年人构成重大风险,特别是那些患有并发病的人.
- 感染SARS-CoV-2可以影响细胞线粒体,可能导致线粒体DNA (mtDNA) 的突变.
研究的目的:
- 在老年COVID-19患者中调查mtDNA中的单核酸替代.
- 在这个人口群体中分析mtDNA变异和炎症生物标志物之间的相关性.
主要方法:
- 在30名COVID-19患者和33名对照人群中,使用基于芯片的复序系统 (MitoChip v2.0) 来测序30名COVID-19患者和33名对照人的mtDNA.
- 对同质细胞和异质细胞mtDNA变异的评估,包括低水平的异质细胞 (<10%).
- 通过高灵敏性免疫测试测量血清炎症标记物 (IL-6,IFN-α,TNF-α,IL-10).
主要成果:
- 与对照组相比,COVID-19患者的总异质体变异体负担较高,ND1和COIII基因显著增加.
- 在COVID-19患者中观察到明显升高的低水平异质体,特别是在呼吸复合体I的基因中.
- 异质体变异负担和低水平的异质体都与IL-6,TNF-α和IFN-α水平的升高有关.
结论:
- 在老年人中,SARS-CoV-2感染可能会诱导mtDNA突变.
- 观察到的mtDNA突变,特别是低水平的异质体,与COVID-19患者的炎症反应的严重程度有关.
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