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开发一个深井板启用高吞吐量 (DEHT) 平台简化了用于生物制造的细胞系开发. 这种自动化系统显著减少了识别理想生产细胞线所需的资源,因为它可以选五倍多的培养.

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科学领域:

  • 生物技术是生物技术.
  • 生物加工是一种生物加工.
  • 细胞线发展发展 细胞线发展

背景情况:

  • 细胞系发育 (CLD) 对于治疗性生物制造至关重要,主要是使用中国仓鼠卵巢 (CHO) 细胞.
  • 随机集成和可塑性等CHO细胞特征导致生产力,生长和质量的显著多样性,需要进行广泛的查.
  • 传统的CLD是劳累和资源密集型,因为需要选多个细胞池和克隆.

研究的目的:

  • 开发和验证一款新型深井板 (DWP) 启用高吞吐量 (DEHT) 平台,用于加速细胞线开发.
  • 评估24井DWP对关键CLD步骤的适用性,包括细胞传递,成像,扩展和料批量生产.
  • 与传统方法相比,证明DEHT平台在选更多种植的效率.

主要方法:

  • 实施一个DEHT CLD平台,利用24井DWP,液体处理器和自动化.
  • 通过评估井对井和板对板的变化和交叉污染来验证24DWP的适用性.
  • 24DWP培养中的生长,产量和产品质量的比较与传统的摇瓶培养.

主要成果:

  • 在24DWP格式中,DEHT平台成功地集成了关键的CLD步骤.
  • 在24DWP培养物中证实了最小的变异性和缺乏交叉污染.
  • 24DWP培养物表现出与震动瓶培养物相比较的生长,产量和产品质量.
  • DEHT平台可以选比传统的CLD多5倍的培养物.

结论:

  • 在生物制造业中,DEHT平台为细胞系开发提供了强大而高效的解决方案.
  • 这种自动化方法大大减少了识别高产细胞系所需的时间和资源.
  • DEHT平台在优化生物制药生产的早期阶段方面取得了重大进展.