在Pseudomonas chlororaphis中开发静态和动态基因表达调节工具包
Sheng-Jie Yue1, Zheng Zhou1, Peng Huang1
1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
研究人员为 Pseudomonas chlororaphis 开发了新的基因表达工具,这种细菌用于工业. 这种工具包可以精确控制基因活动,帮助代谢工程和生物技术应用.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 非模型微生物代谢对于工业生物技术至关重要.
- Pseudomonas chlororaphis 是一种具有工业潜力的促进植物生长的细菌.
- 缺乏精确的基因调节工具限制了P. chlororaphis的应用.
研究的目的:
- 为P. chlororaphis. 构建和描述一个促进物-5'-UTR (PUTR) 库.
- 开发静态和动态的基因表达调节系统.
- 为微调P. chlororaphis. 的基因表达提供一个工具包.
主要方法:
- 构建了108个促进子-5'-UTR (PUTR) 元素的库.
- 使用光蛋白检测和记者基因 (lacZ,phzO) 进行PUTR特征.
- 开发了IPTG可诱导和细胞密度调节的等离子体,用于动态控制.
主要成果:
- 确定了6个具有稳定的低,中,高表达水平的PUTR.
- 建立了一个静态基因表达调节系统.
- 创建了动态基因表达调节系统 (IPTG诱导和自我诱导).
结论:
- 开发了P. chlororaphis. 的两个调控系统 (静态和动态).
- 为精确的基因表达控制提供了有效的工具包.
- 在P. chlororaphis中启用了代谢流量重编程,用于生物技术.
更多相关视频
08:34Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Published on: January 8, 2020
08:57Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Published on: March 10, 2014
相关概念视频
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
