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在Xenopus laevis (FETAX) 的胚胎发生过程中,布洛芬诱导的多器官形

Mae Ja Park¹, Jeong-Pil Chae², Dongju Woo¹

¹Department of Anatomy, School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea.

Biochemical and biophysical research communications

|February 20, 2024

概括

在Xenopus laevis胚胎的发育过程中,易布洛芬暴露导致显著的胃发育延迟和多器官形,以度依赖的方式证明其强烈的胚胎毒性.

科学领域:

发育毒理学发展毒理学
药理学药理学是指药理学的学科.
脊椎动物的胚胎发生

背景情况:

非类固醇抗炎药物 (NSAIDs),如布洛芬,被广泛使用,但它们对脊椎动物的全部胚胎毒性仍未评估.
了解常用药品对早期发育的影响对于公共健康和安全至关重要.

研究的目的:

系统地评估布洛芬对Xenopus laevis胚胎的胚胎毒性作用.
为了确定布洛芬暴露和发育异常之间的剂量依赖关系.

主要方法:

在96小时内,Xenopus laevis胚胎被暴露在不同度的布洛芬 (864毫克/升) 中.
每天检查胚胎是否具有致命和原性影响,并进行组织学和基因表达分析.
实时定量PCR用于评估不同发育阶段器官标记基因的表达.

主要成果:

易布洛芬暴露导致延迟或失败的背唇形成和胃流关闭,导致内皮.
观察到的发育不良包括小头症,发育不良的肠道,以及心脏,肺部和前管的低可塑性.
发育障碍,包括头部,心脏和骨干,以度依赖的方式发生,原性指数为2.28.

结论:

伊布洛芬显著破坏早期胃流动,并在Xenopus laevis胚胎的器官生成过程中引起广泛的器官形.
这些发现凸显了进一步评估ibuprofen在脊椎动物模型中的胚胎毒性潜力的必要性.

关键词:

胚胎毒性胚胎毒性菲塔克斯 (Fetax) 是一个婴儿.伊布罗芬是什么意思伊布罗芬是什么意思多器官形多器官形器官标记器官标记器是指器官的标记器.它们是 Xenopus laevis 的类型.

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