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双光蛋白表达系统检测细胞周期依赖的蛋白质噪声

Akshaya Murugesan¹, Reshod A Alshagrawi², Ramesh Thiyagarajan³

¹Molecular Signaling Group, Faculty of Medicine and Health Technology, Tampere University and BioMediTech, P.O. Box 553, 33101 Tampere, Finland.; Department of Biotechnology, Lady Doak College, Madurai Kamaraj University, Thallakulam, Madurai 625002, India.

International journal of biological macromolecules

|February 20, 2024

概括

由基因表达引起的细胞噪声因细胞周期阶段和诱导剂度而异. 离子影响种群的一致性,表明对蛋白质表达噪声的严格控制.

科学领域:

细胞生物学细胞生物学
系统生物学系统生物学
生物物理学的生物物理.

背景情况:

同样的细胞中的表型变异源于随机基因表达 (噪音).
噪声成分 (内在和外在) 受诱导剂度和细胞周期阶段的影响.
了解噪声调节对于生物过程至关重要.

研究的目的:

量化细胞周期各阶段的噪声成分变化.
研究离子对噪声和人口均性的影响.
为了比较多副本与单副本等离子体系统中的噪声.

主要方法:

在多副本等离子体上使用双色光蛋白记者系统 (CFP/YFP).
诱导了与异烯β-D-1-thiogalactopyranoside (IPTG) 的乳酸促进剂.
多种 (Mg2+) 离子度 (2.5mM和5mM) 和在不同周期阶段分析的细胞.

主要成果:

在多复制和单复制系统之间观察到25%的噪声差异.
外在噪声是总变化的主要因素,从滞后阶段增加到指数阶段,然后下降.
5毫米Mg2+促进了比2.5毫米Mg2+更大的种群同质性.

结论:

蛋白质表达噪声受到细胞周期阶段和细胞内离子度的严格调节.
外在噪音在特定生长阶段显著影响细胞变异.
离子度是控制人口同质性的关键因素.

关键词:

细胞循环的细胞周期噪声组件噪声组件噪声组件活动主办方的动态蛋白质表达方式蛋白质表达方式

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