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在肝纤维化中的功能基因的识别,基于对 lncRNA 中介 ceRNA 网络的生物信息学分析

Feng Zhang^1,2, Siya Pei^2,3, Meifang Xiao^4,5

¹Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Hunan, Changsha, 410008, People's Republic of China.

BMC medical genomics

|February 20, 2024

概括

这项研究确定了肝纤维化的新型调节机制,涉及长非编码RNA (lncRNA) TNFRSF10A-DT. 这种IncRNA可能作为肝纤维化的诊断生物标志物,为其病变产生提供了新的见解.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

肝纤维化是一个重要的全球健康问题,其分子机制尚不清楚.
了解肝纤维化病原体对于开发有效的治疗方法至关重要.

研究的目的:

构建一个lncRNA-miRNA-mRNA网络以识别参与肝纤维化的关键lncRNA.
探索 lncRNAs 在肝纤维化病原发生中的潜在作用.
为了确定肝纤维化的潜在诊断生物标志物.

主要方法:

利用基因表达综合数据库和生物信息学分析来识别肝纤维化组织中差异表达的基因 (DEG).
建立了基于lncRNA,miRNA和mRNA相互作用的竞争性内源RNA (ceRNA) 网络.
进行了功能性丰富分析,并构建了一个蛋白质-蛋白质相互作用 (PPI) 网络.
使用定量实时聚合酶链反应 (qRT-PCR) 验证的关键 lncRNA.

主要成果:

建立了一个包含3个lncRNA,5个miRNA和93个mRNA的ceRNA网络.
功能丰富分析突出了与癌症和细胞成分调节相关的途径.
通过PPI网络和数据库分析,通过PPI网络和数据库分析确定了与肝纤维化相关的7个枢纽基因.
发现 lncRNA AC100861 (lncRNA TNFRSF10A-DT) 在肝纤维化组织和激活的肝星状细胞中显著降低.

结论:

lncRNA TNFRSF10A-DT可以作为肝纤维化诊断和预后的潜在生物标志物.
在肝纤维化病原发生过程中发现了一种新的IncRNA介导的ceRNA调节机制.

关键词:

生物信息学分析肝脏纤维化肝脏纤维化在 Perl 中使用 Perl.ceRNARNA是什么意思在ncRNA中,我们可以

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